Mutagenic insertion and chromosome engineering resource (MICER)

David J. Adams, Patrick J. Biggs, Tony Cox, Rob Davies, Louise Van Der Weyden, Jos Jonkers, James Smith, Bob Plumb, Ruth Taylor, Ichiko Nishijima, Yuejin Yu, Jane Rogers, Allan Bradley

Research output: Contribution to journalArticle

113 Citations (Scopus)

Abstract

Embryonic stem cell technology revolutionized biology by providing a means to assess mammalian gene function in vivo. Although it is now routine to generate mice from embryonic stem cells, one of the principal methods used to create mutations, gene targeting, is a cumbersome process. Here we describe the indexing of 93,960 ready-made insertional targeting vectors from two libraries. 5,925 of these vectors can be used directly to inactivate genes with an average targeting efficiency of 28%. Combinations of vectors from the two libraries can be used to disrupt both alleles of a gene or engineer larger genomic changes such as deletions, duplications, translocations or inversions. These indexed vectors constitute a public resource (Mutagenic Insertion and Chromosome Engineering Resource; MICER) for high-throughput, targeted manipulation of the mouse genome.

Original languageEnglish
Pages (from-to)867-871
Number of pages5
JournalNature Genetics
Volume36
Issue number8
DOIs
Publication statusPublished - 2004 Aug
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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  • Cite this

    Adams, D. J., Biggs, P. J., Cox, T., Davies, R., Van Der Weyden, L., Jonkers, J., Smith, J., Plumb, B., Taylor, R., Nishijima, I., Yu, Y., Rogers, J., & Bradley, A. (2004). Mutagenic insertion and chromosome engineering resource (MICER). Nature Genetics, 36(8), 867-871. https://doi.org/10.1038/ng1388