TY - JOUR
T1 - Mutagenic insertion and chromosome engineering resource (MICER)
AU - Adams, David J.
AU - Biggs, Patrick J.
AU - Cox, Tony
AU - Davies, Rob
AU - Van Der Weyden, Louise
AU - Jonkers, Jos
AU - Smith, James
AU - Plumb, Bob
AU - Taylor, Ruth
AU - Nishijima, Ichiko
AU - Yu, Yuejin
AU - Rogers, Jane
AU - Bradley, Allan
N1 - Funding Information:
We thank W.C. Skarnes, members of the laboratories of W.C. Skarnes and A.B. and the Sanger informatics team for discussions and assistance. D.J.A and L.v.d.W. were supported by a National Health and Medical Research Council CJ Martin fellowship and a National Health and Medical Research Council CJ Martin/RG Menizes fellowship, respectively. J.J. is a recipient of a NWO Genomics Fellowship from the Netherlands Organization for Scientific Research. This work was supported by the Wellcome Trust (UK).
PY - 2004/8
Y1 - 2004/8
N2 - Embryonic stem cell technology revolutionized biology by providing a means to assess mammalian gene function in vivo. Although it is now routine to generate mice from embryonic stem cells, one of the principal methods used to create mutations, gene targeting, is a cumbersome process. Here we describe the indexing of 93,960 ready-made insertional targeting vectors from two libraries. 5,925 of these vectors can be used directly to inactivate genes with an average targeting efficiency of 28%. Combinations of vectors from the two libraries can be used to disrupt both alleles of a gene or engineer larger genomic changes such as deletions, duplications, translocations or inversions. These indexed vectors constitute a public resource (Mutagenic Insertion and Chromosome Engineering Resource; MICER) for high-throughput, targeted manipulation of the mouse genome.
AB - Embryonic stem cell technology revolutionized biology by providing a means to assess mammalian gene function in vivo. Although it is now routine to generate mice from embryonic stem cells, one of the principal methods used to create mutations, gene targeting, is a cumbersome process. Here we describe the indexing of 93,960 ready-made insertional targeting vectors from two libraries. 5,925 of these vectors can be used directly to inactivate genes with an average targeting efficiency of 28%. Combinations of vectors from the two libraries can be used to disrupt both alleles of a gene or engineer larger genomic changes such as deletions, duplications, translocations or inversions. These indexed vectors constitute a public resource (Mutagenic Insertion and Chromosome Engineering Resource; MICER) for high-throughput, targeted manipulation of the mouse genome.
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U2 - 10.1038/ng1388
DO - 10.1038/ng1388
M3 - Article
C2 - 15235602
AN - SCOPUS:3543035792
VL - 36
SP - 867
EP - 871
JO - Nature Genetics
JF - Nature Genetics
SN - 1061-4036
IS - 8
ER -