Mutagenesis targeted by triple-helix forming oligonucleotides containing a reactive nucleoside analogue.

Fumi Nagatsugi, Shigeki Sasaki, Michael M. Seidmen, Paul S. Miller

Research output: Contribution to journalArticlepeer-review

Abstract

Recently, we have demonstrated that 2-amino-6-vinypurine derivative (1) achieves triplex-forming cross-linking with high selectivity toward the cytosine of the G-C target site. In this study, we have investigated the cross-linking as well as induction of point mutations with the TFO incorporating 1 to a target site in a shuttle vector plasmid that replicates in mammalian cells. It was revealed that the TFO bearing 1 introduced mutations at the site of cross-linking. These results have suggested that the selective cross-linking with 1 might be useful for development of new biotechnology for targeted-mutagenesis.

Original languageEnglish
Pages (from-to)31-32
Number of pages2
JournalNucleic acids research. Supplement (2001)
Issue number2
DOIs
Publication statusPublished - 2002

ASJC Scopus subject areas

  • Medicine(all)

Fingerprint Dive into the research topics of 'Mutagenesis targeted by triple-helix forming oligonucleotides containing a reactive nucleoside analogue.'. Together they form a unique fingerprint.

Cite this