Muscle type-specific effect of myostatin deficiency on myogenic regulatory factor expression in adult double-muscled Japanese Shorthorn cattle

Susumu Muroya, Kouichi Watanabe, Shinichiro Hayashi, Masato Miyake, Shigeru Konashi, Youichi Sato, Manabu Takahashi, Shigeki Kawahata, Yoshisato Yoshikawa, Hisashi Aso, Koichi Chikuni, Takahiro Yamaguchi

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

To clarify muscle type-specific effect of myostatin on myogenic regulatory factors (MRFs), we examined mRNA expression of MRFs in five skeletal muscles of normal (NM) and myostatin-deficient double-muscled (DM) adult Japanese Shorthorn cattle by quantitative reverse-transcribed PCR. Among the four MRFs, namely, Myf5, MyoD, myogenin, and MRF4, MyoD expression was different among the muscles of the DM cattle (P < 0.01) but not of the NM cattle. Meanwhile, MyoD expression was significantly elevated only in masseter (MS) muscle in the DM cattle due to the myostatin deficiency (P < 0.05). Myf5 and MRF4 expression in semitendinosus (ST) was higher in the DM than in the NM cattle (P < 0.05). According to analysis of myosin heavy chain (MyHC) isoform expression, more MyHC-2x and -2a and less -slow isoforms were expressed in the longissimus and ST muscles compared to the MS muscle in both cattle (P < 0.05), but no significant difference in MyHC expression was observed between the NM and DM cattle. Taken together, myostatin has influences on Myf5 and MRF4 expression in faster-type muscles and on MyoD expression in slower-type muscles, suggesting a possible muscle type-specific effect of myostatin in skeletal muscle growth and maintenance.

Original languageEnglish
Pages (from-to)678-685
Number of pages8
JournalAnimal Science Journal
Volume80
Issue number6
DOIs
Publication statusPublished - 2009 Dec 1
Externally publishedYes

Keywords

  • MRF4
  • Muscle type
  • Myf5
  • MyoD
  • Myostatin

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

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