This paper describes assembly of three dimensional cellular structure by optical tweezers. We attempted to construct a three dimensional quasi living machine in small space with muscle cells. Cardiomyocytes were manipulated by using laser optical tweezers at wavelength of 1064 nm. The cellular bridge structure was successful constructed on between PDMS micro pillars. The viability and contraction of that was was confirmed. We also demonstrated a nano assembly of a celldriven nano-mechanical tweezers by using a optical tweezers. The nano mechanical tweezers was designed as single cardiomyocyte contraction force for a nano actuator. A myocyte contraction force is tiny force such as <10 μN, however it is enough large power to drive the nano mechanics. This nano mechanics was three-dimensional carbon structure which was designed for nano tweezers. Focused ion beam induced chemical vapor deposition of the carbon nano mechanics. Optical tweezers allowed to combine the nano tweezers and a single beating cardiomyocyte on it. We demonstrated assembly of single cardiomyocyte powered nano tweezers, which had beams of 150 nm diameter and 20 μm length, and live cardiomyocyte.