Murine forebrain and midbrain crest cells generate different characteristic derivatives in vitro

Suconta Chareonvit, Noriko Osumi-Yamashita, Masa Aki Ikeda, Kazuhiro Eto

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

Neural crest (NC) is a transient structure that gives rise to various types of tissues. Many NC cells are pluripotent in the sense that their progeny can generate more than one derivative. However, the potentiality to differentiate into certain derivatives, such as cartilage and bone, seems to be specified with respect to the neuraxial levels at which the NC generates. In order to compare the differentiation potentiality of different regions of head NC, the derivatives of forebrain and midbrain mouse NC have been investigated in vitro using explant cultures of neuroepithelial fragments. From morphology and expression of specific markers, the midbrain crest cultures obviously generated earlier and were greater in number of neuronal cells than were the forebrain ones. Moreover, collagen type II positive cells were detected in the midbrain but not in the forebrain crest cultures. Finally, pigment cells were only observed in the forebrain cultures. The results suggest that the forebrain and midbrain crest cells have a different potentiality to differentiate.

Original languageEnglish
Pages (from-to)493-503
Number of pages11
JournalDevelopment Growth and Differentiation
Volume39
Issue number4
DOIs
Publication statusPublished - 1997
Externally publishedYes

Keywords

  • Differentiation potentiality
  • Forebrain
  • Midbrain
  • Mouse embryo
  • Neural crest culture

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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