TY - JOUR
T1 - Murine central neurons express a novel member of the cdc10/SWI6 motif-containing protein superfamily
AU - Fujigasaki, Hiroto
AU - Song, Si Young
AU - Kobayashi, Takayoshi
AU - Yamakuni, Tohru
N1 - Funding Information:
We thank Drs. Ian Hopkinson and Eijiro Adachi for critical reading of the manuscript and discussion. This work was supported partially by the Research Grant (5B-4) for Nervous and Mental Disorders from the National Ministry of Health and Welfare.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1996/9/1
Y1 - 1996/9/1
N2 - V-1 protein is a novel member of the cdc10/SWI6 motif-containing protein superfamily several members of which have been demonstrated to play crucial roles in the regulation of intracellular signaling. In the present study we examined the distribution of V-1 mRNA in the murine central nervous system (CNS). Northern analysis revealed the expression of V-1 mRNA in various regions of the brain with the following rank order: hippocampus, cerebellum > cerebral cortex, olfactory bulb, medulla oblongata, pons > thalamus. In situ hybridization also showed that V-1 mRNA is widely distributed in various regions of the brain, with parallel expression levels to those revealed by Northern analysis. Immunohistochemical analysis revealed that the V-1 protein exists in various types of neurons, mainly in cell bodies but also in dendrites, axons and possibly in synaptic areas. These expression patterns of the V-1 gene in the murine CNS suggest that the V-1 protein performs some common function in different classes of neurons. We found no significant difference in the expression level of V-1 mRNA in cerebellar granule cells between the control and mutant mice of Purkinje cell degeneration (pcd). In comparison with our previous data obtained in another mutant, staggerer, we discussed the effects of target deprivation on the expression of V-1 mRNA in cerebellar granule cells.
AB - V-1 protein is a novel member of the cdc10/SWI6 motif-containing protein superfamily several members of which have been demonstrated to play crucial roles in the regulation of intracellular signaling. In the present study we examined the distribution of V-1 mRNA in the murine central nervous system (CNS). Northern analysis revealed the expression of V-1 mRNA in various regions of the brain with the following rank order: hippocampus, cerebellum > cerebral cortex, olfactory bulb, medulla oblongata, pons > thalamus. In situ hybridization also showed that V-1 mRNA is widely distributed in various regions of the brain, with parallel expression levels to those revealed by Northern analysis. Immunohistochemical analysis revealed that the V-1 protein exists in various types of neurons, mainly in cell bodies but also in dendrites, axons and possibly in synaptic areas. These expression patterns of the V-1 gene in the murine CNS suggest that the V-1 protein performs some common function in different classes of neurons. We found no significant difference in the expression level of V-1 mRNA in cerebellar granule cells between the control and mutant mice of Purkinje cell degeneration (pcd). In comparison with our previous data obtained in another mutant, staggerer, we discussed the effects of target deprivation on the expression of V-1 mRNA in cerebellar granule cells.
KW - Cellular localization
KW - Gene expression
KW - Murine central nervous system
KW - V-1 protein
KW - cdc10/SWI6 motif
KW - pcd mutant mice
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U2 - 10.1016/0169-328X(96)00005-8
DO - 10.1016/0169-328X(96)00005-8
M3 - Article
C2 - 8872304
AN - SCOPUS:0030249016
VL - 40
SP - 203
EP - 213
JO - Molecular Brain Research
JF - Molecular Brain Research
SN - 0006-8993
IS - 2
ER -