Multiscale Approach to Characterize Mechanical Properties of Tissue Engineered Skin

S. Tupin, J. Molimard, V. Cenizo, T. Hoc, B. Sohm, H. Zahouani

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)


Tissue engineered skin usually consist of a multi-layered visco-elastic material composed of a fibrillar matrix and cells. The complete mechanical characterization of these tissues has not yet been accomplished. The purpose of this study was to develop a multiscale approach to perform this characterization in order to link the development process of a cultured skin to the mechanical properties. As a proof-of-concept, tissue engineered skin samples were characterized at different stages of manufacturing (acellular matrix, reconstructed dermis and reconstructed skin) for two different aging models (using cells from an 18- and a 61-year-old man). To assess structural variations, bi-photonic confocal microscopy was used. To characterize mechanical properties at a macroscopic scale, a light-load micro-mechanical device that performs indentation and relaxation tests was designed. Finally, images of the internal network of the samples under stretching were acquired by combining confocal microscopy with a tensile device. Mechanical properties at microscopic scale were assessed. Results revealed that adding cells during manufacturing induced structural changes, which provided higher elastic modulus and viscosity. Moreover, senescence models exhibited lower elastic modulus and viscosity. This multiscale approach was efficient to characterize and compare skin equivalent samples and permitted the first experimental assessment of the Poisson’s ratio for such tissues.

Original languageEnglish
Pages (from-to)2851-2862
Number of pages12
JournalAnnals of Biomedical Engineering
Issue number9
Publication statusPublished - 2016 Sep 1
Externally publishedYes


  • Bi-photonic confocal microscopy
  • Digital image correlation
  • Indentation
  • Opto-mechanical system
  • Poisson’s ratio
  • Reconstructed skin

ASJC Scopus subject areas

  • Biomedical Engineering


Dive into the research topics of 'Multiscale Approach to Characterize Mechanical Properties of Tissue Engineered Skin'. Together they form a unique fingerprint.

Cite this