Multiplex PCR-based genotyping of mitochondrial DNA from two species of the genus Batillaria (B. multiformis and B. flectosiphonata) from Amami-Oshima, Japan

Kimiko Hirose, Takefumi Yorisue, Hajime Itoh, Tomoko Yamamoto, Shigeaki Kojima

Research output: Contribution to journalArticlepeer-review

Abstract

Snails of the genus Batillaria are common on most muddy tidal flats throughout Japan. To conserve tidal flat ecosystems, it is essential to understand the ecology of the Batillaria snails. A previous mitochondrial (mt) cytochrome c oxidase subunit I (COI) gene sequence analysis indicated that two Batillaria species, B. flectosiphonata and B. multiformis, occur together on the tidal flats on Amami-Oshima, Japan. However, it is difficult to distinguish them by their morphological traits. In this study, a simple multiplex PCR (mPCR)-based method was developed to identify the two species without the need for DNA sequencing. The method allows the discrimination of species by the electrophoretic detection of species-specific DNA fragments of the COI gene amplified by mPCR. This method will enhance the ecological study of these Batillaria snails on Amami-Oshima.

Original languageEnglish
Pages (from-to)67-70
Number of pages4
JournalPlankton and Benthos Research
Volume9
Issue number1
DOIs
Publication statusPublished - 2014 Jan 1
Externally publishedYes

Keywords

  • Amami-Oshima
  • Batillaria
  • Mitochondrial DNA
  • Multiplex PCR
  • Tidal flat ecosystem

ASJC Scopus subject areas

  • Oceanography
  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science
  • Ecology

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