Multiple processing forms and their biological activities of a novel angiogenesis inhibitor vasohibin

Hikaru Sonoda, Hideki Ohta, Kazuhide Watanabe, Hiroshi Yamashita, Hiroshi Kimura, Yasufumi Sato

Research output: Contribution to journalArticlepeer-review

42 Citations (Scopus)

Abstract

Vasohibin is a newly identified negative feedback regulator for angiogenesis. When expressed in cultured human endothelial cells, vasohibin polypeptides were detected in multiple distinct molecular weight forms, suggesting that some proteolytic events may occur within cells or the pericellular milieu. In order to identify the proteolysis sites, vasohibin cDNA mutants were generated to substitute some basic amino acids with alanine and then were transfected into endothelial cells. Western blots with anti-vasohibin monoclonal antibody following the transfection showed that there were at least two cleaving sites in the amino terminal region. Purified recombinant protein of the amino terminal truncated forms not only retained its inhibitory activity on angiogenesis in mouse corneal assay but also showed strong affinity to heparin. Moreover, deletion of some basic residues at the carboxyl terminal resulted in abrogation of both antiangiogenic and heparin-binding activities. Processing patterns and biological activities of the processed forms of this novel antiangiogenic factor are discussed.

Original languageEnglish
Pages (from-to)640-646
Number of pages7
JournalBiochemical and biophysical research communications
Volume342
Issue number2
DOIs
Publication statusPublished - 2006 Apr 7

Keywords

  • Angiogenesis
  • Heparin-binding
  • Processing
  • Vasohibin

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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