Abstract
Heterologous expression of four candidate genes found in ophiobolin gene clusters from three fungal strains was employed to elucidate the late-stage biosynthetic pathway of phytotoxin ophiobolin. Expression of oblBAc (cytochrome P450) from the cryptic gene cluster gave unexpected products, and that of oblBBm/oblBEv from the gene cluster of ophiobolin producers, with oblDBm as the transporter, yielded intermediate ophiobolin C through an unusual four-step oxidation process. The observation made in this study may provide a useful guideline for the elucidation of genuine biosynthetic pathways of natural products.
| Original language | English |
|---|---|
| Pages (from-to) | 1980-1983 |
| Number of pages | 4 |
| Journal | Organic letters |
| Volume | 18 |
| Issue number | 9 |
| DOIs | |
| Publication status | Published - 2016 May 6 |
ASJC Scopus subject areas
- Biochemistry
- Physical and Theoretical Chemistry
- Organic Chemistry
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Narita, K., Chiba, R., Minami, A., Kodama, M., Fujii, I., Gomi, K., & Oikawa, H. (2016). Multiple Oxidative Modifications in the Ophiobolin Biosynthesis: P450 Oxidations Found in Genome Mining. Organic letters, 18(9), 1980-1983. https://doi.org/10.1021/acs.orglett.6b00552