Multiple gene organization of pufferfish Fugu rubripes tropomyosin isoforms and tissue distribution of their transcripts

Takuya Toramoto, Daisuke Ikeda, Yoshihiro Ochiai, Shinsei Minoshima, Nobuyoshi Shimizu, Shugo Watabe

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

The Japanese pufferfish, torafugu (Fugu rubripes), has a haploid genome of about 400 Mb in size, which has been sequenced to approximately 90% coverage. Here we identified six Fugu tropomyosin (TPM) gene sequences by using the BLASTN program and the sequence of the white croaker TPM1 gene in our collection against the draft assembly of the Fugu genomic sequence database. TPM2, TPM3 and TPM4 genes were identified together with a set of two potentially duplicated genes of TPM1 (TPM1-1 and TPM1-2) as described in our previous report and TPM4 (TPM4-1 and TPM4-2) newly found in this study. The expression patterns of these Fugu TPM genes were determined by reverse transcription polymerase chain reaction (RT-PCR). A phylogenetic tree was constructed using the deduced amino acid sequences, which were encoded by the exons common to all vertebrate TPM genes. This indicated that the Fugu TPM1 and TPM4 genes had resulted from a gene duplication in the fish evolutionary lineage.

Original languageEnglish
Pages (from-to)41-51
Number of pages11
JournalGene
Volume331
Issue number1-2
DOIs
Publication statusPublished - 2004 Apr 28
Externally publishedYes

Keywords

  • Alternative splicing
  • EST
  • Expressed sequence tag
  • Fugu
  • Genome duplication
  • HMW
  • High molecular weight
  • LMW
  • Low molecular weight
  • RT-PCR
  • Reverse transcription polymerase chain reaction
  • TPM
  • TnT
  • Tropomyosin
  • Troponin T

ASJC Scopus subject areas

  • Genetics

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