TY - JOUR
T1 - Multiple digit formation in Xenopus limb bud recombinants
AU - Yokoyama, Hitoshi
AU - Endo, Tetsuya
AU - Tamura, Koji
AU - Yajima, Hiroshi
AU - Ide, Hiroyuki
N1 - Funding Information:
We thank Professor K. Muneoka and Professor D. L. Stocum for critically reading the manuscript. We also thank Dr. Kosuke Tash-iro for the gift of Xenopus sonic hedgehog cDNA. This work was supported by research grants from the Ministry of Education, Science and Culture of Japan.
PY - 1998/4/1
Y1 - 1998/4/1
N2 - We prepared recombinant limb buds of Xenopus tadpoles by grafting a mesenchyme mass of the hindlimb bud. The Xenopus recombinant limb buds with dissociated and reaggregated mesenchyme developed more than 30 digits with cartilage segmentation, while those with undissociated mesenchyme developed a limb with normal cartilage pattern. Before the formation of multiple digits, a patchy expression pattern of fgf-8, an AER marker, was observed in the distal region of recombinant limb buds. shh, a ZPA (zone of polarizing activity) marker, was expressed broadly in the distal region of recombinants. Recombinant limb buds with the reaggregated mesenchyme of anterior halves formed anterior digits with claws, and those with the mesenchyme of posterior halves formed posterior digits without claws. The temporal and spatial changes in the potency of multiple digit formation are discussed with reference to the regenerative capacity of Xenopus limb buds.
AB - We prepared recombinant limb buds of Xenopus tadpoles by grafting a mesenchyme mass of the hindlimb bud. The Xenopus recombinant limb buds with dissociated and reaggregated mesenchyme developed more than 30 digits with cartilage segmentation, while those with undissociated mesenchyme developed a limb with normal cartilage pattern. Before the formation of multiple digits, a patchy expression pattern of fgf-8, an AER marker, was observed in the distal region of recombinant limb buds. shh, a ZPA (zone of polarizing activity) marker, was expressed broadly in the distal region of recombinants. Recombinant limb buds with the reaggregated mesenchyme of anterior halves formed anterior digits with claws, and those with the mesenchyme of posterior halves formed posterior digits without claws. The temporal and spatial changes in the potency of multiple digit formation are discussed with reference to the regenerative capacity of Xenopus limb buds.
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U2 - 10.1006/dbio.1998.8856
DO - 10.1006/dbio.1998.8856
M3 - Article
C2 - 9527876
AN - SCOPUS:0032054347
VL - 196
SP - 1
EP - 10
JO - Developmental Biology
JF - Developmental Biology
SN - 0012-1606
IS - 1
ER -