Multiparametric characterization of a single cell by an ultrasound and optical combined microscope

Yoshifumi Saijo, Joe Shikama, Koki Yoshida, Ryo Nagaoka, Mototaka Arakawa, Kazuto Kobayashi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Biomechanics of the cell has been gathering much attention because it affects the pathological status in atherosclerosis and cancer. In the present study, an ultrasound and optical combined microscope system for characterization of a single cell with multiple ultrasound parameters was developed. The central frequency of the transducer was 375 MHz and the scan area was 80 × 80 micron with up to 200 × 200 sampling points. An inversed optical microscope was installed in the system and simultaneous observation of the cells cultured in the petri-dish was available. Two-dimensional mapping of the multiple ultrasound parameters such as attenuation, sound speed and acoustic impedance as well as the thickness and density of the cell was realized by the system. Sound speed and thickness of 3T3-L1 fibroblast cell were successfully obtained by the system. The ultrasound and optical combined microscope system may contribute to understand the cellular biomechanics.

Original languageEnglish
Title of host publicationIEEE International Ultrasonics Symposium, IUS
PublisherIEEE Computer Society
Pages727-730
Number of pages4
ISBN (Electronic)9781479970490
DOIs
Publication statusPublished - 2014 Oct 20
Event2014 IEEE International Ultrasonics Symposium, IUS 2014 - Chicago, United States
Duration: 2014 Sep 32014 Sep 6

Publication series

NameIEEE International Ultrasonics Symposium, IUS
ISSN (Print)1948-5719
ISSN (Electronic)1948-5727

Other

Other2014 IEEE International Ultrasonics Symposium, IUS 2014
CountryUnited States
CityChicago
Period14/9/314/9/6

Keywords

  • acoustic microscopy
  • biomechanics
  • cell
  • sound speed

ASJC Scopus subject areas

  • Acoustics and Ultrasonics

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