Multimodal functional analysis platform: 1. Ultrathin fluorescence endoscope imaging system enables flexible functional brain imaging

Makoto Osanai, Hideki Miwa, Atsushi Tamura, Satomi Kikuta, Yoshio Iguchi, Yuchio Yanagawa, Kazuto Kobayashi, Norihiro Katayama, Tetsu Tanaka, Hajime Mushiake

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

To elucidate the expression mechanisms of brain functions, we have developed an ultrathin fluorescence endoscope imaging system (U-FEIS) that can image cells in the brain at any depth while minimizing the invasion. The endoscope part of U-FEIS consists of a GRIN lens and a 10,000-pixel image fiber with a diameter of 450 μm. The specialized microscope of U-FEIS is within 30 cm square and includes lenses and optical filters optimized for the endoscope. Using U-FEIS, we successfully visualized neurons expressing GFP with single-cell resolution and recorded the multineuronal activities in vitro and in vivo. U-FEIS can also perform imaging and optical stimulation simultaneously. Therefore, U-FEIS should be a powerful optical tool in neuroscience research.

Original languageEnglish
Title of host publicationAdvances in Experimental Medicine and Biology
PublisherSpringer
Pages471-479
Number of pages9
DOIs
Publication statusPublished - 2021

Publication series

NameAdvances in Experimental Medicine and Biology
Volume1293
ISSN (Print)0065-2598
ISSN (Electronic)2214-8019

Keywords

  • Activity imaging
  • Endoscope
  • GRIN lens
  • Image fiber
  • Imaging system
  • Multicellular imaging

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Multimodal functional analysis platform: 1. Ultrathin fluorescence endoscope imaging system enables flexible functional brain imaging'. Together they form a unique fingerprint.

Cite this