Multilayered, core/shell nanoprobes based on magnetic ferric oxide particles and quantum dots for multimodality imaging of breast cancer tumors

Qiang Ma, Yuko Nakane, Yuki Mori, Miyuki Hasegawa, Yoshichika Yoshioka, Tomonobu M. Watanabe, Kohsuke Gonda, Noriaki Ohuchi, Takashi Jin

Research output: Contribution to journalArticlepeer-review

76 Citations (Scopus)

Abstract

Multilayered, core/shell nanoprobes (MQQ-probe) based on magnetic nanoparticles (MNPs) and quantum dots (QDs) have been successfully developed for multimodality tumor imaging. This MQQ-probe contains Fe3O4 MNPs, visible-fluorescent QDs (600 nm emission) and near infrared-fluorescent QDs (780 nm emission) in multiple silica layers. The fabrication of the MQQ-probe involves the synthesis of a primer Fe3O4 MNPs/SiO2 core by a reverse microemulsion method. The MQQ-probe can be used both as a fluorescent probe and a contrast reagent of magnetic resonance imaging. For breast cancer tumor imaging, anti-HER2 (human epidermal growth factor receptor 2) antibody was conjugated to the surface of the MQQ-probe. The specific binding of the antibody conjugated MQQ-probe to the surface of human breast cancer cells (KPL-4) was confirmed by fluorescence microscopy and fluorescence-activated cell sorting analysis in vitro. Due to the high tissue permeability of near-infrared (NIR) light, NIR fluorescence imaging of the tumor mice (KPL-4 cells transplanted) was conducted by using the anti-HER2 antibody conjugated MQQ-probe. In vivo multimodality images of breast tumors were successfully taken by NIR fluorescence and T2-weighted magnetic resonance. Antibody conjugated MQQ-probes have great potential to use for multimodality imaging of cancer tumors in vitro and in vivo.

Original languageEnglish
Pages (from-to)8486-8494
Number of pages9
JournalBiomaterials
Volume33
Issue number33
DOIs
Publication statusPublished - 2012 Nov

Keywords

  • Breast cancer tumor
  • MRI
  • Magnetic nanoparticle
  • Multimodality imaging
  • NIR
  • Quantum dot

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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