Multilayer membranes via layer-by-layer deposition of glucose oxidase and Au nanoparticles on a Pt electrode for glucose sensing

Tomonori Hoshi, Naoki Sagae, Kenji Daikuhara, Kei Takahara, Jun ichi Anzai

Research output: Contribution to journalArticlepeer-review

26 Citations (Scopus)

Abstract

We prepared multilayer membranes by the layer-by-layer deposition of glucose oxidase (GOx) and Au nanoparticles (5, 10, or 50 nm φ) on sensor substrates, such as a Pt electrode and a quartz glass plate, to prepare glucose sensors. The enzyme activity of GOx remained even in alternate assemblies, and the activity increased with the increasing number of depositions. The apparent Km values of the deposited GOx were 28-32 mM, while a reported value in a solution is 33 mM. These results suggest that Au nanoparticles can be used as binders for the deposition of GOx without significant change in the affinity between GOx and glucose.

Original languageEnglish
Pages (from-to)890-894
Number of pages5
JournalMaterials Science and Engineering C
Volume27
Issue number4
DOIs
Publication statusPublished - 2007 May 16

Keywords

  • Glucose oxidase
  • Glucose sensor
  • Gold nanoparticle
  • Layer-by-layer deposition

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanics of Materials
  • Mechanical Engineering

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