Multilayer membranes via layer-by-layer deposition of ascorbate oxidase and Au nanoparticles on the Pt electrode for reduction of oxidation current derived from ascorbate

Tomonori Hoshi, Naoki Sagae, Kenji Daikuhara, Junichi Anzai

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

A glass plate was alternately immersed in an Au colloid (10 nm φ) and an ascorbate oxidase (AOx) solution (0.1 mg/mL, pH 6.8). Absorbance at 530 nm originating from the Au particles increased with the increasing number of depositions. The AOx activity of the plate also increased as the plate was immersed in the AOx solution. These results suggested that a multilayer membrane via the layer-by-layer deposition of AOx and Au nanoparticles was formed on the glass plate. AOx was also deposited on a Pt disk electrode using the same process. Using the (AOx/Au)10 modified electrode, the oxidation current of ascorbic acid (0.1 mM) decreased to 19% versus the unmodified electrode.

Original languageEnglish
Pages (from-to)644-647
Number of pages4
JournalTalanta
Volume71
Issue number2
DOIs
Publication statusPublished - 2007 Feb 15

Keywords

  • Ascorbate oxidase
  • Ascorbic acid
  • Gold nanoparticle
  • Layer-by-layer deposition

ASJC Scopus subject areas

  • Analytical Chemistry

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