Multichannel incubation type planar patch clamp biosensor using plastic (PMMA) substrates and characterization by laser-gated ion-channel protein

Zhi Hong Wang, Hidetaka Uno, Noriko Takada, Obuliraju Senthil Kumar, Toru Ishizuka, Hiromu Yawo, Tsuneo Urisu

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    Abstract

    Recently we have developed 4 channel incubation type planar patch clamp biosensor with polymethylmethacrylate (PMMA) substrate by using both-side hot embossing, focused ion beam micro-fabrication and surface modification by extra cellular matrix. We have evaluated the performance by using the light-gated ion-channel protein, channelrhodopsin wide receiver (ChRWR) expressed on HEK293 cell. Observed laser-induced current profile and the membrane potential dependences well agreed to those observed by pipette patch clamp, suggesting its excellent possibility in important applications such as portable ion channel biosensors and high throughput screening with neural systems.

    Original languageEnglish
    Title of host publicationProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
    PublisherChemical and Biological Microsystems Society
    Pages1033-1035
    Number of pages3
    ISBN (Print)9780979806452
    Publication statusPublished - 2012 Jan 1
    Event16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 - Okinawa, Japan
    Duration: 2012 Oct 282012 Nov 1

    Publication series

    NameProceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

    Other

    Other16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
    CountryJapan
    CityOkinawa
    Period12/10/2812/11/1

    Keywords

    • Focused ion beam
    • HEK293
    • Ion channel current
    • Lithography
    • Multichannel
    • PMMA
    • Patterned planar patch clamp biosensor

    ASJC Scopus subject areas

    • Bioengineering
    • Chemical Engineering (miscellaneous)

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