@inproceedings{1dbf761053e24f64a628389ebd1936ec,
title = "Multichannel incubation type planar patch clamp biosensor using plastic (PMMA) substrates and characterization by laser-gated ion-channel protein",
abstract = "Recently we have developed 4 channel incubation type planar patch clamp biosensor with polymethylmethacrylate (PMMA) substrate by using both-side hot embossing, focused ion beam micro-fabrication and surface modification by extra cellular matrix. We have evaluated the performance by using the light-gated ion-channel protein, channelrhodopsin wide receiver (ChRWR) expressed on HEK293 cell. Observed laser-induced current profile and the membrane potential dependences well agreed to those observed by pipette patch clamp, suggesting its excellent possibility in important applications such as portable ion channel biosensors and high throughput screening with neural systems.",
keywords = "Focused ion beam, HEK293, Ion channel current, Lithography, Multichannel, PMMA, Patterned planar patch clamp biosensor",
author = "Wang, {Zhi Hong} and Hidetaka Uno and Noriko Takada and Kumar, {Obuliraju Senthil} and Toru Ishizuka and Hiromu Yawo and Tsuneo Urisu",
year = "2012",
language = "English",
isbn = "9780979806452",
series = "Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012",
publisher = "Chemical and Biological Microsystems Society",
pages = "1033--1035",
booktitle = "Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012",
note = "16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 ; Conference date: 28-10-2012 Through 01-11-2012",
}