Multibudded tubules formed by COPII on artificial liposomes

Kirsten Bacia, Eugene Futai, Simone Prinz, Annette Meister, Sebastian Daum, Daniela Glatte, John A.G. Briggs, Randy Schekman

    Research output: Contribution to journalArticlepeer-review

    65 Citations (Scopus)

    Abstract

    COPII-coated vesicles form at the endoplasmic reticulum for cargo transport to the Golgi apparatus. We used in vitro reconstitution to examine the roles of the COPII scaffold in remodeling the shape of a lipid bilayer. Giant Unilamellar Vesicles were examined using fast confocal fluorescence and cryo-electron microscopy in order to avoid separation steps and minimize mechanical manipulation. COPII showed a preference for high curvature structures, but also sufficient flexibility for binding to low curvatures. The COPII proteins induced beads-on-a-string-like constricted tubules, similar to those previously observed in cells. We speculate about a mechanical pathway for vesicle fission from these multibudded COPII-coated tubules, considering the possibility that withdrawal of the Sar1 amphipathic helix upon GTP hydrolysis leads to lipid bilayer destabilization resulting in fission.

    Original languageEnglish
    Article number17
    JournalScientific reports
    Volume1
    DOIs
    Publication statusPublished - 2011

    ASJC Scopus subject areas

    • General

    Fingerprint

    Dive into the research topics of 'Multibudded tubules formed by COPII on artificial liposomes'. Together they form a unique fingerprint.

    Cite this