Multi-channel 3-D cell culture device integrated on a silicon chip for anticancer drug sensitivity test

Yu Suke Torisawa, Hitoshi Shiku, Tomoyuki Yasukawa, Matsuhiko Nishizawa, Tomokazu Matsue

Research output: Contribution to journalArticlepeer-review

104 Citations (Scopus)

Abstract

A novel three-dimensional cell culture system was constructed with an array of cell panels (4×5) in a silicon chip, together with multi-channel drug containers. Human breast cancer (MCF-7) cells were embedded in a collagen-gel matrix and entrapped in a pyramidal-shaped silicon hole. Each cell panel can be isolated by a channel composed of a microfluid part and a reservoir. A cell panel was exposed to 200mM KCN for 2 days to demonstrate that each cell panel could be independently evaluated under various stimulation conditions. Based on the cellular respiration activity, the proliferation behavior was continuously monitored on the silicon-based cell array for 5 days using scanning electrochemical microscopy (SECM). The cells entrapped in the device (3-D culture) proliferated normally, and the proliferation rate was lower than that of cells grown in a monolayer cell culture (2-D culture). The effects of three anticancer drugs measured simultaneously on the cell chip were in good agreement with those obtained by a conventional colorimetric assay. Our results suggest that the silicon-based device for 3D culture is appropriate for a chemosensitivity assay involving multi-chemical stimulation.

Original languageEnglish
Pages (from-to)2165-2172
Number of pages8
JournalBiomaterials
Volume26
Issue number14
DOIs
Publication statusPublished - 2005 May 1

Keywords

  • Biosensor
  • Collagen
  • Cytotoxicity
  • Electrochemistry
  • Polydimethylsiloxane

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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