TY - JOUR
T1 - MS-818 accelerates mobilization of endothelial progenitor cells and differentiation to endothelial cells
AU - Kanemura, Masanori
AU - Abe, Mayumi
AU - Ueda, Masatsugu
AU - Ueki, Minoru
AU - Awaya, Akira
AU - Sato, Yasufumi
PY - 2004/9
Y1 - 2004/9
N2 - MS-818 that is a synthetic pyrimidine compound and shown to have neurotrophic actions, enhanced basic fibroblast growth factor (bFGF)-induced angiogenesis in vivo. However, the mechanism and whether MS-818 affects endothelial cells (ECs) directly is not known. Here, the authors investigated whether MS-818 alone could induce angiogenesis and tried to clarify the mechanism of neovascularization by MS-818 in terms of angiogenesis and vasculogenesis. The authors show that MS-818 affects ECs directly and induces migration of and tube formation by ECs in vitro (angiogenesis). Furthermore, the authors demonstrate that MS-818 mobilizes endothelial progenitor cells (EPCs) from the bone marrow and potentiates their differentiation to ECs (vasculogenesis). The effect of MS-818 on the endothelial differentiation was further confirmed with an in vitro differentiation system using mouse embryonic stem cells. MS-818 activates the mitogen-activated protein kinase (MAPK) pathway but not the phosphoinositol 3-kinase (PI3K)-Akt pathway in ECs. These results indicate that MS-818, a synthetic compound, promotes both angiogenesis and vasculogenesis.
AB - MS-818 that is a synthetic pyrimidine compound and shown to have neurotrophic actions, enhanced basic fibroblast growth factor (bFGF)-induced angiogenesis in vivo. However, the mechanism and whether MS-818 affects endothelial cells (ECs) directly is not known. Here, the authors investigated whether MS-818 alone could induce angiogenesis and tried to clarify the mechanism of neovascularization by MS-818 in terms of angiogenesis and vasculogenesis. The authors show that MS-818 affects ECs directly and induces migration of and tube formation by ECs in vitro (angiogenesis). Furthermore, the authors demonstrate that MS-818 mobilizes endothelial progenitor cells (EPCs) from the bone marrow and potentiates their differentiation to ECs (vasculogenesis). The effect of MS-818 on the endothelial differentiation was further confirmed with an in vitro differentiation system using mouse embryonic stem cells. MS-818 activates the mitogen-activated protein kinase (MAPK) pathway but not the phosphoinositol 3-kinase (PI3K)-Akt pathway in ECs. These results indicate that MS-818, a synthetic compound, promotes both angiogenesis and vasculogenesis.
KW - Angiogenesis
KW - Embryonic stem cells (ES Cells)
KW - Endothelial progenitor cells (EPCs)
KW - MS-818
KW - Vasculogenesis
UR - http://www.scopus.com/inward/record.url?scp=12344251726&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=12344251726&partnerID=8YFLogxK
U2 - 10.1080/10623320490904089
DO - 10.1080/10623320490904089
M3 - Article
C2 - 15763941
AN - SCOPUS:12344251726
VL - 11
SP - 221
EP - 230
JO - Endothelium: Journal of Endothelial Cell Research
JF - Endothelium: Journal of Endothelial Cell Research
SN - 1062-3329
IS - 5-6
ER -