MS-818 accelerates mobilization of endothelial progenitor cells and differentiation to endothelial cells

Masanori Kanemura, Mayumi Abe, Masatsugu Ueda, Minoru Ueki, Akira Awaya, Yasufumi Sato

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

MS-818 that is a synthetic pyrimidine compound and shown to have neurotrophic actions, enhanced basic fibroblast growth factor (bFGF)-induced angiogenesis in vivo. However, the mechanism and whether MS-818 affects endothelial cells (ECs) directly is not known. Here, the authors investigated whether MS-818 alone could induce angiogenesis and tried to clarify the mechanism of neovascularization by MS-818 in terms of angiogenesis and vasculogenesis. The authors show that MS-818 affects ECs directly and induces migration of and tube formation by ECs in vitro (angiogenesis). Furthermore, the authors demonstrate that MS-818 mobilizes endothelial progenitor cells (EPCs) from the bone marrow and potentiates their differentiation to ECs (vasculogenesis). The effect of MS-818 on the endothelial differentiation was further confirmed with an in vitro differentiation system using mouse embryonic stem cells. MS-818 activates the mitogen-activated protein kinase (MAPK) pathway but not the phosphoinositol 3-kinase (PI3K)-Akt pathway in ECs. These results indicate that MS-818, a synthetic compound, promotes both angiogenesis and vasculogenesis.

Original languageEnglish
Pages (from-to)221-230
Number of pages10
JournalEndothelium: Journal of Endothelial Cell Research
Volume11
Issue number5-6
DOIs
Publication statusPublished - 2004 Sep 1

Keywords

  • Angiogenesis
  • Embryonic stem cells (ES Cells)
  • Endothelial progenitor cells (EPCs)
  • MS-818
  • Vasculogenesis

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

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