Purpose: To detect the expression of mRNA of protooncogenes ets-1, c-jun, c-fos, and platelet-derived growth factor (PDGF) in proliferative membranes of patients with proliferative diabetic retinopathy (PDR) and proliferative vitreoretinopathy (PVR). Methods: cDNA was synthesized from mRNA of proliferative membranes from patients with PDR, PVR, premacular fibrosis (PMF), acute retinal necrosis (ARN), and age-related macular degeneration (ARMD). Ets-1, c-jun, c-fos, PDGF-A, and PDGF-B cDNA were amplified using reverse transcriptase-polymerase chain reaction (PCR). Results: Proto-oncogene mRNA was highly expressed in membranes from patients with severe PDR, grade D PVR. PDGF mRNA was expressed in almost all samples. Conclusions: Our results suggest that not only PDGF mRNA was expressed in the membranes of patients with PDR and PVR, but proto-oncogenes ets-1, c-jun, and c-fos mRNA were also expressed. Copyright (C) 2000 Japanese Ophthalmological Society.
- Platelet-derived growth factor
- Proliferative diabetic retinopathy
- Proliferative vitreoretinopathy
ASJC Scopus subject areas