TY - JOUR
T1 - MPC polymer regulates fibrous tissue formation by modulating cell adhesion to the biomaterial surface
AU - Ye, Zhang
AU - Hiroyasu, Kanetaka
AU - Yuya, Sano
AU - Kano, Mitsuhiro
AU - Tada-Aki, Kudo
AU - Shimizu, Yoshinaka
PY - 2010
Y1 - 2010
N2 - The aim of this study was to analyze the effects of 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer on fibrous tissue formation and cell adhesion plaque (CAP)-forming reactions. Silastic elastomer (SE) plates coated (experimental group) and uncoated (control group) with MPC polymer were prepared for in vivo and in vitro experiments. For the in vivo animal experiments, SE plates were implanted subcutaneously in the rat dorsal region. At 4, 8, and 12 weeks, thicknesses of the fibrous tissue capsules in the experimental group were lower than in the control group. Likewise, the amount of collagen in the experimental group was lower than that of the control group. For the in vitro cell culture experiments, KMST-6 fibroblast cells in the experimental group demonstrated enhanced cell migration, accompanied with a weaker expression of vinculin and a larger amount of filopodia. Furthermore, weaker expressions of paxillin, talin, and ROCK1, but stronger expression of cofilin, were observed in the experimental group. Taken together, these results suggested that MPC polymer regulated fibrous tissue formation by modulating cell adhesion through changes in local CAPs and downstream signaling.
AB - The aim of this study was to analyze the effects of 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer on fibrous tissue formation and cell adhesion plaque (CAP)-forming reactions. Silastic elastomer (SE) plates coated (experimental group) and uncoated (control group) with MPC polymer were prepared for in vivo and in vitro experiments. For the in vivo animal experiments, SE plates were implanted subcutaneously in the rat dorsal region. At 4, 8, and 12 weeks, thicknesses of the fibrous tissue capsules in the experimental group were lower than in the control group. Likewise, the amount of collagen in the experimental group was lower than that of the control group. For the in vitro cell culture experiments, KMST-6 fibroblast cells in the experimental group demonstrated enhanced cell migration, accompanied with a weaker expression of vinculin and a larger amount of filopodia. Furthermore, weaker expressions of paxillin, talin, and ROCK1, but stronger expression of cofilin, were observed in the experimental group. Taken together, these results suggested that MPC polymer regulated fibrous tissue formation by modulating cell adhesion through changes in local CAPs and downstream signaling.
KW - 2-methacryloyloxyethyl phosphorylcholine polymer
KW - Cell adhesion plaque
KW - Fibrous tissue
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U2 - 10.4012/dmj.2009-138
DO - 10.4012/dmj.2009-138
M3 - Article
C2 - 20827031
AN - SCOPUS:78149385595
VL - 29
SP - 518
EP - 528
JO - Dental Materials Journal
JF - Dental Materials Journal
SN - 0287-4547
IS - 5
ER -