Mouse LIM-kinase 2 gene: cDNA cloning, genomic organization, and tissue- specific expression of two alternatively initiated transcripts

Chiho Ikebe, Kazumasa Ohashi, Toshihiko Fujimori, Ora Bernard, Tetsuo Noda, Elizabeth J. Robertson, Kensaku Mizuno

Research output: Contribution to journalArticlepeer-review

31 Citations (Scopus)

Abstract

LIM-kinase 1 and LIM-kinase 2 (LIMK1 and LIMK2) are members of a novel protein kinase subfamily containing LIM motifs at the N-terminus. There are two isoforms of Limk2 transcripts coding proteins with distinct N-terminal structures: LIMK2a, containing two LIM motifs, and LIMK2b, with one and one- half LIM motifs. Here we report the cDNA and genomic structures of mouse LIMK2. Th deduced 638-amino-acid sequence of mouse LIMK2a shows 98% identity with that of rat LIMK2a. The mouse Limk2a gene consists of at least 16 exons and spans more than 50 kb. Exon/intron boundaries of the mouse Limk2a gene are exactly conserved with those of the mouse Limk1 gene. An additional exon encoding the Limk2b-specific 5'-terminal sequence was found to be located between exons 2 and 3, suggesting that Limk2a and 2b mRNAs are transcribed from a single Limk2 gene by an alternative usage of exons near the 5' end of the gene. Limk2a and Limk2b transcripts were expressed at different ratios in a variety of mouse tissues.

Original languageEnglish
Pages (from-to)504-508
Number of pages5
JournalGenomics
Volume46
Issue number3
DOIs
Publication statusPublished - 1997 Dec 15
Externally publishedYes

Keywords

  • Gene structure
  • LIM domain
  • LIMK2
  • Protein kinase

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'Mouse LIM-kinase 2 gene: cDNA cloning, genomic organization, and tissue- specific expression of two alternatively initiated transcripts'. Together they form a unique fingerprint.

Cite this