Mouse coq7/clk-1 orthologue rescued slowed rhythmic behavior and extended life span of clk-1 longevity mutant in Caenorhabditis elegans

Mayumi Takahashi, Sunao Asaumi, Shuji Honda, Yo ichi Suzuki, Daisuke Nakai, Hidehito Kuroyanagi, Takahiko Shimizu, Yoko Honda, Takuji Shirasawa

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

The coq7/clk-1 gene was isolated from the long-lived mutant of Caenorhabditis elegans and was suggested to play a regulatory role in biological rhythm and longevity. The mouse COQ7 is homologous to Saccharomyces cerevisiae COQ7/CAT5 that is required for the biosynthesis of coenzyme Q (ubiquinone), an essential messenger in mitochondrial respiration. In the present study, we characterized the expression and processing of mouse COQ7. We found that COQ7 is highly expressed in tissues with high energy demand such as heart, muscle, liver, and kidney in mice. Biochemical analysis revealed that COQ7 is targeted to mitochondria where it is processed to mature form. Transgenic expression of mouse coq7 completely rescued the slowed rhythmic behaviors of clk-1 such as defecation. In life-span analysis, transgenic expression reverted the extended life span of clk-1 to the comparable level with wild-type control. These data strongly suggested that coq7 plays a pivotal role in the regulation of biological rhythms and the determination of life span in mammalian species.

Original languageEnglish
Pages (from-to)534-540
Number of pages7
JournalBiochemical and biophysical research communications
Volume286
Issue number3
DOIs
Publication statusPublished - 2001

Keywords

  • Caenorhabditis elegans
  • Coq7/clk-1
  • Life span
  • Longevity
  • Mitochondria
  • Mouse
  • Rhythm
  • Ubiquinone

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Fingerprint Dive into the research topics of 'Mouse coq7/clk-1 orthologue rescued slowed rhythmic behavior and extended life span of clk-1 longevity mutant in Caenorhabditis elegans'. Together they form a unique fingerprint.

Cite this