Motoneuron degeneration after facial nerve avulsion is exacerbated in presymptomatic transgenic rats expressing human mutant Cu/Zn superoxide dismutase

Ken Ikeda, Masashi Aoki, Yoko Kawazoe, Tsuyoshi Sakamoto, Yuichi Hayashi, Aya Ishigaki, Makiko Nagai, Rieko Kamii, Shinsuke Kato, Yasuto Itoyama, Kazuhiko Watabe

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

We investigated motoneuron degeneration after proximal nerve injury in presymptomatic transgenic (tg) rats expressing human mutant Cu/Zn superoxide dismutase (SOD1). The right facial nerves of presymptomatic tg rats expressing human H46R or G93A SOD1 and their non-tg littermates were avulsed, and facial nuclei were examined at 2 weeks postoperation. Nissl-stained cell counts revealed that facial motoneuron loss after avulsion was exacerbated in H46R- and G93A-tg rats compared with their non-tg littermates. The loss of motoneurons in G93A-tg rats after avulsion was significantly greater than that in H46R-tg rats. Intense cytoplasmic immunolabeling for SOD1 in injured motoneurons after avulsion was demonstrated in H46R- and G93A-tg rats but not in their littermates. Facial axotomy did not induce significant motoneuron loss nor enhance SOD1 immunoreactivity in these tg rats and non-tg littermates at 2 weeks postoperation, although both axotomy and avulsion elicited intense immunolabeiing for activating transcription factor-3, phosphorylated c-Jun, and phosphorylated heat shock protein 27 in injured motoneurons of all these animals. The present data indicate the increased vulnerability of injured motoneurons after avulsion in the presymptomatic mutant SOD1-tg rats.

Original languageEnglish
Pages (from-to)63-70
Number of pages8
JournalJournal of Neuroscience Research
Volume82
Issue number1
DOIs
Publication statusPublished - 2005 Oct 1

Keywords

  • ALS
  • Amyotrophic lateral sclerosis
  • Axotomy
  • Facial nerve
  • Mutant Cu/Zn superoxide dismutase
  • SOD1
  • Transgenic rat

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

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