Motility of spirochetes

Shuichi Nakamura, Md Shafiqul Islam

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

Spirochetes are bacteria distinguished by an undulate or helical cell body and intracellular flagellar called periplasmic flagella or endoflagella. Spirochetes translate by rotating the cell body. In this chapter, we show a method for simultaneous measurement of the cell body rotation and swimming speed in individual spirochete cells. We also describe a simple chemotaxis assay capable of observing the response of spirochete in real time under a microscope and quantitatively evaluating the response magnitude to attractants and repellents.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages243-251
Number of pages9
DOIs
Publication statusPublished - 2017 Jan 1

Publication series

NameMethods in Molecular Biology
Volume1593
ISSN (Print)1064-3745

Keywords

  • Chemotaxis
  • Microscopic agar-drop assay
  • One-sided dark-field microscope
  • Spirochetes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Nakamura, S., & Islam, M. S. (2017). Motility of spirochetes. In Methods in Molecular Biology (pp. 243-251). (Methods in Molecular Biology; Vol. 1593). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6927-2_19