Motility of spirochetes

Shuichi Nakamura, Md Shafiqul Islam

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Citations (Scopus)


Spirochetes are bacteria distinguished by an undulate or helical cell body and intracellular flagellar called periplasmic flagella or endoflagella. Spirochetes translate by rotating the cell body. In this chapter, we show a method for simultaneous measurement of the cell body rotation and swimming speed in individual spirochete cells. We also describe a simple chemotaxis assay capable of observing the response of spirochete in real time under a microscope and quantitatively evaluating the response magnitude to attractants and repellents.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Number of pages9
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Chemotaxis
  • Microscopic agar-drop assay
  • One-sided dark-field microscope
  • Spirochetes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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