Motility of single one-headed kinesin molecules along microtubules

Yuichi Inoue, Atsuko Hikikoshi Iwane, Takayuki Miyai, Etsuko Muto, Toshio Yanagida

Research output: Contribution to journalArticlepeer-review

54 Citations (Scopus)

Abstract

The motility of single one-headed kinesin molecules (K351 and K340), which were truncated fragments of Drosophila two-headed kinesin, has been tested using total internal reflection fluorescence microscopy. One-headed kinesin fragments moved continuously along the microtubules. The maximum distance traveled until the fragments dissociated from the microtubules for both K351 and K340 was ∼600 nm. This value is considerably larger than the space resolution of the measurement system (SD ≈ 30 nm). Although the movements of the fragments fluctuated in forward and backward directions, statistical analysis showed that the average movements for both K340 and K351 were toward the plus of the microtubules, i.e., forward direction. When BDTC (a 1.3-S subunit of Propionibacterium shermanii transcarboxylase, which binds weakly to a microtubule), was fused to the tail (C-terminus) of K351, its movement was enhanced, smooth, and unidirectional, similar to that of the two-headed kinesin fragment, K411. However, the travel distance and velocity of K351BDTC molecules were ∼3-fold smaller than that of K411. These observations suggest that a single kinesin head has basal motility, but coordination between the two heads is necessary for stabilizing the basal motility for the normal level of kinesin processivity.

Original languageEnglish
Pages (from-to)2838-2850
Number of pages13
JournalBiophysical Journal
Volume81
Issue number5
DOIs
Publication statusPublished - 2001

ASJC Scopus subject areas

  • Biophysics

Fingerprint

Dive into the research topics of 'Motility of single one-headed kinesin molecules along microtubules'. Together they form a unique fingerprint.

Cite this