Morphology of the granular hemocytes of the Japanese horseshoe crab Tachypleus tridentatus and immunocytochemical localization of clotting factors and antimicrobial substances

Yoshihiro Toh, Akiko Mizutani, Fuminori Tokunaga, Tatsushi Muta, Sadaaki Iwanaga

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64 Citations (Scopus)

Abstract

The structure of hemocytes in the normal state and during blood coagulation, and the intracellular localization of three clotting factors and two antimicrobial factors were examined in the Japanese horseshoe crab Tachypleus tridentatus. Two types of hemocytes were found in the circulating blood: non-granular and granular hemocytes. The latter contained numerous dense granules classed into two major types: L- and D-granules. The L-granules were larger (up to 1.5 μm in diameter) and less electron-dense than the D-granules (less than 0.6 μm in diameter). The L-granules contained three clotting factors and one antimicrobial factor, whereas the D-granules exclusively contained the other antimicrobial factor. After treatment with endotoxin, the L-granules were released more rapidly than the D-granules, although almost all granules were finally exocytosed. The granular hemocyte possessed a single Golgi complex; possible precursor granules of L-granules and D-granules contained tubular and condensed dense material, respectively. These data are discussed in relation to the self-defense mechanisms of the horseshoe crab.

Original languageEnglish
Pages (from-to)137-147
Number of pages11
JournalCell and Tissue Research
Volume266
Issue number1
DOIs
Publication statusPublished - 1991 Oct 1

Keywords

  • Antimicrobial substances
  • Clotting factors
  • Hemocytes
  • Immunocytochemistry
  • Tachypleus tridentatus (Chelicerata)

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

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