TY - JOUR
T1 - Morphologies of Vesicle Doublets
T2 - Competition among Bending Elasticity, Surface Tension, and Adhesion
AU - Murakami, Kei
AU - Ebihara, Ryuta
AU - Kono, Takuma
AU - Chiba, Toshikaze
AU - Sakuma, Yuka
AU - Ziherl, Primož
AU - Imai, Masayuki
N1 - Funding Information:
This work was in part supported by Japan Society for the Promotion of Science (Grant Numbers 25247070 , 16H02216 , 17K14368 , 20K03873 , 20H00120 and KAKENHI “Fluctuation and Structure" Grant Number 25103009 ). The authors also acknowledge the financial support from the Slovenian Research Agency (research core funding No. P1-0055 and project No. J2-9223).
Publisher Copyright:
© 2020 Biophysical Society
PY - 2020/11/3
Y1 - 2020/11/3
N2 - To study the mechanical laws governing the form of multicellular organisms, we examine the morphology of adhering vesicle doublets as the simplest model system. We monitor the morphological transformations of doublets induced by changes of adhesion strength and volume/area ratio, which are controlled by intermembrane interactions and thermal area expansion, respectively. When we increase the temperature in the weak adhesion regime, a dumbbell flat-contact doublet is transformed to a parallel-prolate doublet, whereas in the strong adhesion regime, heating transforms the dumbbell flat-contact doublet into a spherical sigmoid-contact doublet. We reproduce the observed doublet morphologies by numerically minimizing the total energy, including the contact-potential adhesion term as well as the surface and bending terms, using the Surface Evolver package. From the reproduced morphologies, we extract the adhesion strength, the surface tension, and the volume/area ratio of the vesicles, which reveals the detailed mechanisms of the morphological transitions in doublets.
AB - To study the mechanical laws governing the form of multicellular organisms, we examine the morphology of adhering vesicle doublets as the simplest model system. We monitor the morphological transformations of doublets induced by changes of adhesion strength and volume/area ratio, which are controlled by intermembrane interactions and thermal area expansion, respectively. When we increase the temperature in the weak adhesion regime, a dumbbell flat-contact doublet is transformed to a parallel-prolate doublet, whereas in the strong adhesion regime, heating transforms the dumbbell flat-contact doublet into a spherical sigmoid-contact doublet. We reproduce the observed doublet morphologies by numerically minimizing the total energy, including the contact-potential adhesion term as well as the surface and bending terms, using the Surface Evolver package. From the reproduced morphologies, we extract the adhesion strength, the surface tension, and the volume/area ratio of the vesicles, which reveals the detailed mechanisms of the morphological transitions in doublets.
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U2 - 10.1016/j.bpj.2020.09.030
DO - 10.1016/j.bpj.2020.09.030
M3 - Article
C2 - 33080225
AN - SCOPUS:85093667924
VL - 119
SP - 1735
EP - 1748
JO - Biophysical Journal
JF - Biophysical Journal
SN - 0006-3495
IS - 9
ER -