TY - JOUR
T1 - Morphological responses of cultured bovine aortic endothelial cells to fluid-imposed shear stress under sparse and colony conditions
AU - Kataoka, Noriyuki
AU - Ujita, Shingo
AU - Kimura, Keishu
AU - Sato, Masaaki
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1997
Y1 - 1997
N2 - The morphological responses of cultured bovine aortic endothelial cells to fluid-imposed shear stress under sparse and colony conditions were studied. Applied shear stress was controlled to 2 Pa and the exposure time was set to be between 1 and 24 hours. Before and after flow exposure, the shape index and the angle of cell orientation to flow direction of each individual cell were measured. In the experiment under sparse conditions, the shape index was 0.33±0.13 (mean±SD) and cells almost randomly distributed under the no-flow condition. This randomness continued following application of shear stress for 24 hours, and a morphological difference between the cells under no-flow conditions and after exposure to shear stress was not observed. In the experiment under colony conditions, the cells in the center portion of the colony were round and were randomly oriented under no-flow conditions. After exposure to shear stress for 24 hours, the cells in the center portion were elongated and aligned with the flow direction, although the cells on the periphery were randomly oriented. From these results, we conclude that the degree of cell to cell contact may affect the morphological response of endothelial cells to fluid-imposed shear stress.
AB - The morphological responses of cultured bovine aortic endothelial cells to fluid-imposed shear stress under sparse and colony conditions were studied. Applied shear stress was controlled to 2 Pa and the exposure time was set to be between 1 and 24 hours. Before and after flow exposure, the shape index and the angle of cell orientation to flow direction of each individual cell were measured. In the experiment under sparse conditions, the shape index was 0.33±0.13 (mean±SD) and cells almost randomly distributed under the no-flow condition. This randomness continued following application of shear stress for 24 hours, and a morphological difference between the cells under no-flow conditions and after exposure to shear stress was not observed. In the experiment under colony conditions, the cells in the center portion of the colony were round and were randomly oriented under no-flow conditions. After exposure to shear stress for 24 hours, the cells in the center portion were elongated and aligned with the flow direction, although the cells on the periphery were randomly oriented. From these results, we conclude that the degree of cell to cell contact may affect the morphological response of endothelial cells to fluid-imposed shear stress.
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U2 - 10.1299/kikaic.63.838
DO - 10.1299/kikaic.63.838
M3 - Article
AN - SCOPUS:0031096748
VL - 63
SP - 838
EP - 845
JO - Nihon Kikai Gakkai Ronbunshu, C Hen/Transactions of the Japan Society of Mechanical Engineers, Part C
JF - Nihon Kikai Gakkai Ronbunshu, C Hen/Transactions of the Japan Society of Mechanical Engineers, Part C
SN - 0387-5024
IS - 607
ER -