Morphological reproductive characteristics of testes and fertilization capacity of cryopreserved sperm after the Fukushima accident in raccoon (Procyon lotor)

Kazuki Komatsu, Tsugumi Iwasaki, Kosuke Murata, Hideaki Yamashiro, Valerie Swee Ting Goh, Ryo Nakayama, Yohei Fujishima, Takumi Ono, Yasushi Kino, Yoshinaka Simizu, Atsushi Takahashi, Hisashi Shinoda, Kentaro Ariyoshi, Kosuke Kasai, Masatoshi Suzuki, Maria Grazia Palmerini, Manuel Belli, Guido Macchiarelli, Toshitaka Oka, Manabu FukumotoMitsuaki A. Yoshida, Akifumi Nakata, Tomisato Miura

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Since the Fukushima Daiichi Nuclear Power Plant (FDNPP) accident, we have established an archive system of livestock and wild animals from the surrounding ex-evacuation zone. Wildlife within the alert zone have been exposed to low-dose-rate (LDR) radiation for a long continuous time. In this study, we analysed the morphological characteristics of the testes and in vitro fertilization (IVF) capacity of cryopreserved sperm of racoons from the ex-evacuation zone of the FDNPP accident. The radioactivity of caesium-137 (137Cs) was measured by gamma-ray spectrometry, and the measured radioactivity concentration was 300–6,630 Bq/kg in the Fukushima raccoons. Notably, normal spermatogenesis was observed in the seminiferous tubules of the testes, with the germinal epithelium composed of a spermatogenic cell lineage with no evident ultrastructural alterations; freeze-thawing sperm penetration ability was confirmed using the interspecific zona pellucida-free mouse oocytes IVF assays. This study revealed that the chronic and LDR radiation exposure associated with the FDNPP accident had no adverse effect on the reproductive characteristics and functions of male raccoons.

Original languageEnglish
Pages (from-to)484-497
Number of pages14
JournalReproduction in Domestic Animals
Volume56
Issue number3
DOIs
Publication statusPublished - 2021 Mar

Keywords

  • biobank
  • environmental pollution
  • gonad
  • procyonids
  • wildlife

ASJC Scopus subject areas

  • Biotechnology
  • Animal Science and Zoology
  • Endocrinology

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