Purpose: To follow the morphological changes in the retina of a transgenic mouse line carrying a mutant phosducin gene which prevents it from being phosphorylated. Earlier studies showed that the transgenic mice showed photoreceptor degeneration. Methods: Transgenic mice were constructed in which serine 73 of the phosducin gene was replaced by isoleucine. Light and electron microscopy and immunocytochemistry were used to follow the changes in the retina. Results: Early Stage; photoreceptors normal, RPE unusual numbers of residual bodies. Intermed. Stage; vacuolization of OS with some of discs oriented vertically, pyknotic nuclei in ONL. Late Stage: OS shortened and distorted, ONL=2-3 rows. The IS of the transgenics appear to have more free ribosomes. Immunocytochemistry: Staining confined to IS of photoreceptors, ONL and OPL of both controls and transgenics but staining is significantly stronger in the transgenics. Conclusions: These data demonstrate that the mutant phosoducin gene will lead to higher levels of phosducin-immunoreactivity in the transgenics and eventually to photoreceptor degeneration.
|Journal||Investigative Ophthalmology and Visual Science|
|Publication status||Published - 1996 Feb 15|
ASJC Scopus subject areas
- Sensory Systems
- Cellular and Molecular Neuroscience