Morphological and gene expression analysis under cool temperature conditions in rice anther development

Susumu Oda, Fumi Kaneko, Kentaro Yano, Tomoaki Fujioka, Hiromi Masuko, Jong In Park, Shunsuke Kikuchi, Kazuki Hamada, Makoto Endo, Kuniaki Nagano, Yoshiaki Nagamura, Makiko Kawagishi-Kobayashi, Keita Suwabe, Go Suzuki, Masao Watanabe

    Research output: Contribution to journalArticle

    22 Citations (Scopus)

    Abstract

    Cool temperature conditions are known to lead to pollen sterility in rice. Pollen sterility is an agriculturally important phenomenon because it imparts a large influence directly on rice yield. However, cool temperature stress tolerance varies among rice cultivars and avoidance of cool temperature stress is difficult by prac- tical method of agriculture. In this study using two rice cultivars, Hitomebore (high tolerance) and Sasanishiki (low tolerance), we analyzed morphological fea- tures and gene expression profiles, under cool temperature stress, in anther devel- opment of rice. Hitomebore was given cool temperature stress (19°C) at flowering stage, and showed 87.3% seed fertility. Meanwhile, the seed fertility decreased to 41.7% in the case of Sasanishiki. A transverse section of Hitomebore anther revealed that the degradation of the tapetum started at the uninucleate microspore stage, and the tapetum had completely vanished at mature stage. The tapetum provides nutrients for pollen development, and its degradation occurs at a late stage in pollen development. In contrast, degradation of the tapetum did not occur at the uninucleate microspore stage in Sasanishiki, and the tapetum was clearly intact at mature stage, suggesting that tapetum degradation is critical for accurate pollen development and cool temperature tolerance correlated with the degree of tapetum degeneration. In gene expression analysis of anther, 356 genes that showed different expression levels between two cultivars at cool temperatures were found. These genes will lead to understanding the mechanism of cool temperature stress response in rice pollen development and the identification of genes involved in accurate tapetum degradation.

    Original languageEnglish
    Pages (from-to)107-120
    Number of pages14
    JournalGenes and Genetic Systems
    Volume85
    Issue number2
    DOIs
    Publication statusPublished - 2010 Jun 29

    Keywords

    • Cool temperature stress
    • Microarray
    • Oryza sativa l.
    • Quantitative trait locus (qtl)
    • Tapetum

    ASJC Scopus subject areas

    • Molecular Biology
    • Genetics

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  • Cite this

    Oda, S., Kaneko, F., Yano, K., Fujioka, T., Masuko, H., Park, J. I., Kikuchi, S., Hamada, K., Endo, M., Nagano, K., Nagamura, Y., Kawagishi-Kobayashi, M., Suwabe, K., Suzuki, G., & Watanabe, M. (2010). Morphological and gene expression analysis under cool temperature conditions in rice anther development. Genes and Genetic Systems, 85(2), 107-120. https://doi.org/10.1266/ggs.85.107