Monosialyl-Gb5 organized with cSrc and FAK in GEM of human breast carcinoma MCF-7 cells defines their invasive properties

Wim F. Steelant, Yasushi Kawakami, Akihiro Ito, Kazuko Handa, Erik A. Bruyneel, Marc Mareel, Senitiroh Hakomori

Research output: Contribution to journalArticlepeer-review

42 Citations (Scopus)

Abstract

Two human mammary carcinoma cell variants, MCF-7/AZ and MCF-7/6, show the same composition in their glycosphingolipid-enriched microdomain (GEM) with regard to globo-series structures Gb3, Gb4, Gb5, monosialyl-Gb5, GM2, and cSrc and FAK. Both variants are non-invasive into collagen gel layer, and showed similar motility in wound migration assay. Whereas invasiveness and motility of MCF-7/AZ cells were enhanced greatly by treatment with mAb RM1 directed to monosialyl-Gb5, the same RM1 treatment had no effect on MCF-7/6. cSrc and FAK of MCF-7/AZ, but not MCF-7/6, were activated by RM1 treatment. Thus, malignancy of MCF-7 is highly dependent on monosialyl-Gb5, and its activation of cSrc and FAK in GEM.

Original languageEnglish
Pages (from-to)93-98
Number of pages6
JournalFEBS Letters
Volume531
Issue number1
DOIs
Publication statusPublished - 2002 Oct 30
Externally publishedYes

Keywords

  • Activation
  • CD9
  • Collagen gel
  • Glycosphingolipid
  • Invasion
  • Microdomain
  • Sensor
  • Signal transducer
  • Tumor cell motility

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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