Monoclonal antibody directed to human T-cell malignancy antigen

Masayoshi Minegishi, Shigeru Tsuchiya, Naoko Minegishi, Osamu Yoshie, Tasuke Konno

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

A murine monoclonal antibody (B2D) against a cultured pre-T acute lymphoblastic leukemia (ALL) cell line THP-6 has been produced. The antibody reacted with seven out of eight cultured T-ALL cell lines and with leukemic cells from three out of four T-ALL/lymphoma patients. The antibody did not react with normal T and B lymphocytes, monocytes, granulocytes, platelets, erythrocytes, bone marrow lymphoid-like precursor cells, thymocytes and other acute and chronic leukemic cells of non-T cell origin. Furthermore, B2D did not react with phytohemagglutinin-activated T cells nor with concanavalin A-activated T cells. The molecules immunoprecipitated with B2D had molecular weights of 50-55 kD. Thus, B2D seems to be highly specific for T-cell malignancies. These results show that B2D defines one of human leukemia antigens which are expressed on the cell surface of T-ALL cells. Monoclonal antibody B2D may be useful for the subclassification of T-ALL cells and has therapeutic potential for a certain type of T-ALL.

Original languageEnglish
Pages (from-to)43-51
Number of pages9
JournalLeukemia Research
Volume13
Issue number1
DOIs
Publication statusPublished - 1989

Keywords

  • T-cell malignancy
  • monoclonal antibody
  • tumor cell antigen

ASJC Scopus subject areas

  • Hematology
  • Oncology
  • Cancer Research

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