TY - JOUR
T1 - Monoclonal antibodies to various morphologic components of human skin
AU - Aiba, S.
AU - Masuko, T.
AU - Hosokawa, M.
AU - Hashimoto, Y.
N1 - Funding Information:
The advent of t he monoclonal antibody technique by K ohler and Milstein (9) facilitated the studies on definite antigenic structures and antigen distributions in a variety of cells or tissues of experimental animals and human. Recently, Woodcock-Mitchell et al [10) have prepared monoclonal antibodies against human keratin and obtained 3 different monoclonal antibodies t hat were reactive with either basal layer, suprabasal Manuscript received April 25, 1983; accepted for publication July 14, 1983 Supported in part by Grants-in-Aid for Cancer from the Ministry of Health and Welfare, Japan. Reprint requests to: Dr. Yoshiyuki Hashimoto, Department of Hygienic Sciences, Pharmaceutical Institute, Tohoku University, Aobayama, Sendai 980, Japan. Abbreviations: ELISA: enzyme-linked immunosorbent assay FBS: fetal bovine serum FITC: fluorescein isothiocyanate HBSS: Hanks' balanced salt solution IIF: indirect immunofluorescence MEM: Eagle's minimum essential medium MoAb: monoclonal antibody(ies) PBS: phosphate-buffered saline layer, or the entire epidermis. In order to extend the monoclonal technique to the detection of skin-associated antigens, we prepared monoclona l antibodies to different components of human skin tissues. This paper communicates the preparation of 8 monoclonal antibodies reactive with different morphologic components of human skin.
PY - 1983
Y1 - 1983
N2 - Somatic cell hybrids were established from the mouse myeloma, P3x63Ag8.653 cells, and the spleen cells of a mouse hyperimmune to human epidermal cells. Indirect immunofluorescence test with hybridoma culture fluids displayed that 253 out of 263 hybridoma cultures secreted antibodies reactive with the frozen sections of human skin. The hybridomas secreting unique antibodies to skin components were cloned and designated as AHS-1 to -8. Monoclonal antibodies (MoAb) from AHS-1, AHS-2, and AHS-3 hybridomas did detect cytoplasmic antigens present in the epidermal layer, eccrine ducts and glands (except MoAb AHS-1), and hair follicles. Enzyme-linked immunosorbent assay showed that the antigen recognized by either MoAb AHS-1 or MoAb AHS-2, but not by MoAb AHS-3, shares the antigenic determinant with antigen(s) present in keratin polypeptides isolated from human callus. MoAb AHS-4 to -8 distinctly stained each morphologic component of the skin tissues: cell membranes of epidermal cells by MoAb AHS-4, cytoplasms in upper layer cells of epidermis by MoAb AHS-5, dermis by MoAb AHS-6, basement membrane zone by MoAb AHS-7, and eccrine ducts by MoAb AHS-8.
AB - Somatic cell hybrids were established from the mouse myeloma, P3x63Ag8.653 cells, and the spleen cells of a mouse hyperimmune to human epidermal cells. Indirect immunofluorescence test with hybridoma culture fluids displayed that 253 out of 263 hybridoma cultures secreted antibodies reactive with the frozen sections of human skin. The hybridomas secreting unique antibodies to skin components were cloned and designated as AHS-1 to -8. Monoclonal antibodies (MoAb) from AHS-1, AHS-2, and AHS-3 hybridomas did detect cytoplasmic antigens present in the epidermal layer, eccrine ducts and glands (except MoAb AHS-1), and hair follicles. Enzyme-linked immunosorbent assay showed that the antigen recognized by either MoAb AHS-1 or MoAb AHS-2, but not by MoAb AHS-3, shares the antigenic determinant with antigen(s) present in keratin polypeptides isolated from human callus. MoAb AHS-4 to -8 distinctly stained each morphologic component of the skin tissues: cell membranes of epidermal cells by MoAb AHS-4, cytoplasms in upper layer cells of epidermis by MoAb AHS-5, dermis by MoAb AHS-6, basement membrane zone by MoAb AHS-7, and eccrine ducts by MoAb AHS-8.
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U2 - 10.1111/1523-1747.ep12522742
DO - 10.1111/1523-1747.ep12522742
M3 - Article
C2 - 6196419
AN - SCOPUS:0021016134
VL - 81
SP - 498
EP - 502
JO - Journal of Investigative Dermatology
JF - Journal of Investigative Dermatology
SN - 0022-202X
IS - 6
ER -