Monoclonal Antibodies Inhibiting Invasion of Cultured Cells by Eimeria tenella Sporozoites

Tatsuya Uchida, Katsuaki Kikuchi, Hiroshi Takano, Keiji Ogimoto, Yutaka Nakai

    Research output: Contribution to journalArticlepeer-review

    6 Citations (Scopus)

    Abstract

    Monoclonal antibodies (MoAbs) secreted by 12 hybridomas and reactive with the surface antigens of E. tenella sporozoites were produced. These MoAbs designated as KC-1 to KC-12 were characterized as IgG3K. In Western blot analysis, these MoAbs reacted with only one polypeptide band of sporozoite antigens having a molecular mass of 25 kDa. All the MoAbs were reactive with sporozoites. trophozoites, immature and mature first generation schizonts, first generation merozoites, and the interior structure of sporulating oocysts of E. tenella, however, not with any of the methanol-fixed sporozoites of E. acervulina, E. mitis, E. maxima, E. brunetti, E. necatrix and E. praecox. Invasion of primary culture of chicken kidney cells by E. tenella sporozoites was significantly inhibited by several of the MoAbs. These results suggest that these MoAbs recognizing the E. tenella-specific surface molecule are involved in the invasion of sporozoites into host cells.

    Original languageEnglish
    Pages (from-to)721-723
    Number of pages3
    JournalJournal of Veterinary Medical Science
    Volume59
    Issue number8
    DOIs
    Publication statusPublished - 1997 Aug

    Keywords

    • Cell invasion
    • Eimeria tenella
    • Monoclonal antibody

    ASJC Scopus subject areas

    • veterinary(all)

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