Monitoring the expression profiles of genes induced by hyperosmotic, high salinity, and oxidative stress and abscisic acid treatment in Arabidopsis cell culture using a full-length cDNA microarray

Seiji Takahashi, Motoaki Seki, Junko Ishida, Masakazu Satou, Tetsuya Sakurai, Mari Narusaka, Asako Kamiya, Maiko Nakajima, Akiko Enju, Kenji Akiyama, Kazuko Yamaguchi-Shinozaki, Kazuo Shinozaki

Research output: Contribution to journalArticlepeer-review

108 Citations (Scopus)

Abstract

Transcriptional regulation in response to hyperosmotic, high-salinity and oxidative stress, and abscisic acid (ABA) treatment in Arabidopsis suspension-cultured cell line T87 was investigated with a cDNA microarray containing 7000 independent full-length Arabidopsis cDNAs. The transcripts of 102, 11, 84 and 73 genes were increased more than 5-fold within 5 h after treatment with 0.5 M mannitol, 0.1 M NaCl, 50 μM ABA and 10 mM H 2O2, respectively. On the other hand, the transcripts of 44, 57, 25 and 34 genes were down-regulated to less than one-third within 5 h after treatment with 0.5 M mannitol, 0.1 M NaCl, 50 μM ABA and 10 mM H 2O2, respectively. Venn diagram analysis revealed 11 genes were induced significantly by mannitol, NaCl, and ABA, indicating crosstalk among these signaling pathways. Comparison of the genes induced by each stress revealed that 32%, 17% and 33% of mannitol-, NaCl- and ABA-inducible genes were also induced by H2O2, indicating the crosstalk between the signaling pathways for osmotic stress and oxidative stress. Although the expression profiles revealed that the T87 cells had most of the regulatory systems seen in Arabidopsis seedlings, the T87 cells did not have one of ABA-dependent signaling pathways.

Original languageEnglish
Pages (from-to)29-55
Number of pages27
JournalPlant Molecular Biology
Volume56
Issue number1
DOIs
Publication statusPublished - 2004 Sep 1

Keywords

  • Abiotic stress
  • Arabidopsis thaliana
  • Full-length cDNA
  • T87 cell culture
  • cDNA microarray

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

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