Monitoring the cellular activity of a cultured single cell by scanning electrochemical microscopy (SECM). A comparison with fluorescence viability monitoring

Takatoshi Kaya, Yu Suke Torisawa, Daisuke Oyamatsu, Matsuhiko Nishizawa, Tomokazu Matsue

Research output: Contribution to journalArticlepeer-review

74 Citations (Scopus)

Abstract

The respiratory activities of cultured HeLa cells were monitored at a single cell level using scanning electrochemical microscopy (SECM) that produces images of the localized distribution of oxygen around the cell. The change in the cellular activity was traced after exposures to KCN, ethyl alcohol and the antibiotic drug, Antimycin A. The results were compared with those from the conventional fluorescence monitoring using Calcein-AM that is sensitive to deformation of the cell membrane. The SECM-based measurement follows the decrease in the cellular activity upon exposure to KCN and Antimycin A more rapidly than the fluorescence-based measurements, demonstrating that SECM is suitable for studying the cellular influence of respiration inhibitors.

Original languageEnglish
Pages (from-to)1379-1383
Number of pages5
JournalBiosensors and Bioelectronics
Volume18
Issue number11
DOIs
Publication statusPublished - 2003 Oct 1

Keywords

  • Calcein
  • Cytotoxicity
  • Respiration activity
  • Scanning electrochemical microscopy (SECM)

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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