Monitoring oxygen consumption of single mouse embryos using an integrated electrochemical microdevice

Yasumoto Date, Shinichiro Takano, Hitoshi Shiku, Kosuke Ino, Takahiro Ito-Sasaki, Masaki Yokoo, Hiroyuki Abe, Tomokazu Matsue

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

Oxygen consumption (respiration activity) has been found to be the most remarkable criterion for determining the viability of an embryo produced in vitro. In this study, we propose an accurate, simple, and user-friendly device for measurement of the oxygen consumption of single mammalian embryos. An integrated electrode array was fabricated to determine the oxygen consumption of a single embryo, including the blastocyst stage, which has an inhomogeneous oxygen consumption rate, using a single measurement procedure. A single mouse embryo was positioned in a microwell at the center of an integrated electrode array, using a mouthpiece pipette, and immobilized by a cylindrical micropit with good reproducibility. The oxygen consumption of two-cell, morula, and blastocyst stages was measured amperometrically using the device. The recorded current profile was corrected to take into consideration transient background current during the measurement. A calculation method for oxygen consumption based on spherical diffusion centered on the defined point of the device was developed. This procedure is quite simple because it is not necessary to estimate the radius of the embryo being measured. The calculated values of oxygen consumption for two-cell, morula, and blastocyst stages were 1.36±0.33×10 -15mols -1, 1.38±0.58×10 -15mols -1, and 3.44±2.07×10 -15mols -1, respectively. The increasing pattern of oxygen consumption from morula to blastocyst agreed well with measurements obtained using conventional scanning electrochemical microscopy (SECM).

Original languageEnglish
Pages (from-to)100-106
Number of pages7
JournalBiosensors and Bioelectronics
Volume30
Issue number1
DOIs
Publication statusPublished - 2011 Dec 15

Keywords

  • Electrochemical device
  • Embryo
  • Micro-TAS
  • Respiration activity

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

Fingerprint Dive into the research topics of 'Monitoring oxygen consumption of single mouse embryos using an integrated electrochemical microdevice'. Together they form a unique fingerprint.

Cite this