TY - JOUR
T1 - Monitoring oxygen consumption of single mouse embryos using an integrated electrochemical microdevice
AU - Date, Yasumoto
AU - Takano, Shinichiro
AU - Shiku, Hitoshi
AU - Ino, Kosuke
AU - Ito-Sasaki, Takahiro
AU - Yokoo, Masaki
AU - Abe, Hiroyuki
AU - Matsue, Tomokazu
N1 - Funding Information:
This research is partly supported by the Cabinet Office, Government of Japan , through its “Funding Program for Next Generation World-Leading Researchers” (H.S.); Special Coordination Funds for Promoting Science and Technology, Formation of Innovation Center for Fusion of Advanced Technologies, from the Japan Science and Technology Agency ; and also a Grant-in-Aid for Scientific Research ( 22245011 , 21685008 , and 21106502 ).
PY - 2011/12/15
Y1 - 2011/12/15
N2 - Oxygen consumption (respiration activity) has been found to be the most remarkable criterion for determining the viability of an embryo produced in vitro. In this study, we propose an accurate, simple, and user-friendly device for measurement of the oxygen consumption of single mammalian embryos. An integrated electrode array was fabricated to determine the oxygen consumption of a single embryo, including the blastocyst stage, which has an inhomogeneous oxygen consumption rate, using a single measurement procedure. A single mouse embryo was positioned in a microwell at the center of an integrated electrode array, using a mouthpiece pipette, and immobilized by a cylindrical micropit with good reproducibility. The oxygen consumption of two-cell, morula, and blastocyst stages was measured amperometrically using the device. The recorded current profile was corrected to take into consideration transient background current during the measurement. A calculation method for oxygen consumption based on spherical diffusion centered on the defined point of the device was developed. This procedure is quite simple because it is not necessary to estimate the radius of the embryo being measured. The calculated values of oxygen consumption for two-cell, morula, and blastocyst stages were 1.36±0.33×10 -15mols -1, 1.38±0.58×10 -15mols -1, and 3.44±2.07×10 -15mols -1, respectively. The increasing pattern of oxygen consumption from morula to blastocyst agreed well with measurements obtained using conventional scanning electrochemical microscopy (SECM).
AB - Oxygen consumption (respiration activity) has been found to be the most remarkable criterion for determining the viability of an embryo produced in vitro. In this study, we propose an accurate, simple, and user-friendly device for measurement of the oxygen consumption of single mammalian embryos. An integrated electrode array was fabricated to determine the oxygen consumption of a single embryo, including the blastocyst stage, which has an inhomogeneous oxygen consumption rate, using a single measurement procedure. A single mouse embryo was positioned in a microwell at the center of an integrated electrode array, using a mouthpiece pipette, and immobilized by a cylindrical micropit with good reproducibility. The oxygen consumption of two-cell, morula, and blastocyst stages was measured amperometrically using the device. The recorded current profile was corrected to take into consideration transient background current during the measurement. A calculation method for oxygen consumption based on spherical diffusion centered on the defined point of the device was developed. This procedure is quite simple because it is not necessary to estimate the radius of the embryo being measured. The calculated values of oxygen consumption for two-cell, morula, and blastocyst stages were 1.36±0.33×10 -15mols -1, 1.38±0.58×10 -15mols -1, and 3.44±2.07×10 -15mols -1, respectively. The increasing pattern of oxygen consumption from morula to blastocyst agreed well with measurements obtained using conventional scanning electrochemical microscopy (SECM).
KW - Electrochemical device
KW - Embryo
KW - Micro-TAS
KW - Respiration activity
UR - http://www.scopus.com/inward/record.url?scp=80755153661&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80755153661&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2011.08.037
DO - 10.1016/j.bios.2011.08.037
M3 - Article
C2 - 21955755
AN - SCOPUS:80755153661
VL - 30
SP - 100
EP - 106
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
SN - 0956-5663
IS - 1
ER -