Monitoring of the genetic variability of the hatchery and recaptured fish in the stock enhancement program of the rare species barfin flounder Verasper moseri

María Del Mar Ortega-Villaizán Romo, Shigenori Suzuki, Minoru Ikeda, Masamichi Nakajima, Nobuhiko Taniguchi

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

In relation to the stock enhancement program for barfin flounder, hatchery juveniles produced in 2001 were genotyped using microsatellite DNA markers (msDNA) and then released to natural waters. Subsequently, recaptured individuals, designated as 'tentative recaptured', were examined using msDNA. In order to evaluate the effectiveness of the stock enhancement program, pairwise FST and genic differentiation tests were used to estimate the genetic divergence between the wild samples, the hatchery broodstock, and the tentative recaptured samples. Analysis showed sig-nificant differentiation among these three groups. Pedigree determination by msDNA was used to establish the origin of the tentative recaptured individuals, in order to elucidate whether they were hatchery produced, 'real recaptured', or wild specimens. Wild individuals were not found. The effective population size of the real recaptured stock was very low (Ne = 16.6). Equal family survivability was observed between the released and real recaptured stocks, indicating that the genetic variability of the released stock was maintained in the natural environment. Future broodstock management, breeding designs, and family contribution equalization of the offspring to be released will be required in this rare species to avoid unintended genetic differentiation between the wild population and the hatchery broodstock.

Original languageEnglish
Pages (from-to)1120-1130
Number of pages11
JournalFisheries Science
Volume71
Issue number5
DOIs
Publication statusPublished - 2005 Oct 1

Keywords

  • Effective population size
  • Hatchery stock
  • Pedigree tracing
  • Rare species
  • Recaptured fish
  • Stock enhancement
  • Survival rate

ASJC Scopus subject areas

  • Aquatic Science

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