Monitoring capillary endothelial cell culture and capillary formation in a microdevice by impedance spectroscopy measurements

A. K. Rissanen, S. Ostrovidov, E. Lennon, V. Senez, J. Kim, B. Kim, K. Sakai Furukawa, T. Ushida, Y. Sakai, T. Fujii

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Citations (Scopus)

Abstract

Capillary endothelial cells (ECs) were cultured on gold electrodes in polydimethlysiloxane (PDMS)/glass microdevices. Our objective was to monitor the culture growth by electrical impedance spectroscopy (EIS) measurements, and to observe whether the formation of capillary-like structures in the cell layer produces a change in the impedance spectra. In our contribution, we present a novel micro-device, which enables long-term cell culture and the monitoring of the cell layer properties. We illustrate the usefulness of EIS measurements in observing several culture conditions (confluence, cell detachment, cell death, capillary formation) by providing impedance measurement curves, as well as cell layer pictures of the culture area.

Original languageEnglish
Title of host publication2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology
Pages201-204
Number of pages4
DOIs
Publication statusPublished - 2005
Externally publishedYes
Event2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology - Oahu, HI, United States
Duration: 2005 May 122005 May 15

Publication series

Name2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology
Volume2005

Other

Other2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology
Country/TerritoryUnited States
CityOahu, HI
Period05/5/1205/5/15

ASJC Scopus subject areas

  • Engineering(all)

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