Monitoring capillary endothelial cell culture and capillary formation in a microdevice by impedance spectroscopy measurements

A. K. Rissanen, S. Ostrovidov, E. Lennon, V. Senez, J. Kim, B. Kim, K. Sakai Furukawa, T. Ushida, Y. Sakai, T. Fujii

    Research output: Chapter in Book/Report/Conference proceedingConference contribution

    2 Citations (Scopus)

    Abstract

    Capillary endothelial cells (ECs) were cultured on gold electrodes in polydimethlysiloxane (PDMS)/glass microdevices. Our objective was to monitor the culture growth by electrical impedance spectroscopy (EIS) measurements, and to observe whether the formation of capillary-like structures in the cell layer produces a change in the impedance spectra. In our contribution, we present a novel micro-device, which enables long-term cell culture and the monitoring of the cell layer properties. We illustrate the usefulness of EIS measurements in observing several culture conditions (confluence, cell detachment, cell death, capillary formation) by providing impedance measurement curves, as well as cell layer pictures of the culture area.

    Original languageEnglish
    Title of host publication2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology
    Pages201-204
    Number of pages4
    DOIs
    Publication statusPublished - 2005
    Event2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology - Oahu, HI, United States
    Duration: 2005 May 122005 May 15

    Publication series

    Name2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology
    Volume2005

    Other

    Other2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology
    CountryUnited States
    CityOahu, HI
    Period05/5/1205/5/15

    ASJC Scopus subject areas

    • Engineering(all)

    Fingerprint Dive into the research topics of 'Monitoring capillary endothelial cell culture and capillary formation in a microdevice by impedance spectroscopy measurements'. Together they form a unique fingerprint.

  • Cite this

    Rissanen, A. K., Ostrovidov, S., Lennon, E., Senez, V., Kim, J., Kim, B., Sakai Furukawa, K., Ushida, T., Sakai, Y., & Fujii, T. (2005). Monitoring capillary endothelial cell culture and capillary formation in a microdevice by impedance spectroscopy measurements. In 2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology (pp. 201-204). [1548426] (2005 3rd IEEE/EMBS Special Topic Conference on Microtechnology in Medicine and Biology; Vol. 2005). https://doi.org/10.1109/MMB.2005.1548426