Monitoring Activity of Living Cells Entrapped in a Membrane-Sealed Microcapillary with Scanning Electrochemical Microscopy

Hitoshi Shiku; Takuo Shiraishi; Hiroaki Ohya; Tomokazu Matsue; Hiroyuki Abe; Hiroyoshi Hoshi; Masato Kobayashi

doi:10.5796/electrochemistry.68.890

Monitoring Activity of Living Cells Entrapped in a Membrane-Sealed Microcapillary with Scanning Electrochemical Microscopy

Hitoshi Shiku, Takuo Shiraishi, Hiroaki Ohya, Tomokazu Matsue, Hiroyuki Abe, Hiroyoshi Hoshi, Masato Kobayashi

Promotion Center of Strategic Innovation

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4 Citations (Scopus)

Abstract

The respiration- or photosynthesis-induced change of oxygen concentration was monitored with scanning electrochemical microscopy (SECM). Cell suspension was put into a glass capillary tube and then one edge was sealed with an oxygen permeable membrane. The total volume of the cell suspension in the capillary was as small as 10 μL. The edge of the sealed capillary was imaged with SECM based on oxygen reduction current. The images indicated the respiration activity as low as 2.0×10³ HeLa-S3 cells. This method was also applied for imaging the respiration and photosynthesis activities of a single alga (Bryopsis plumosa) protoplast.

Original language	English
Pages (from-to)	890-892
Number of pages	3
Journal	Electrochemistry
Volume	68
Issue number	11
DOIs	https://doi.org/10.5796/electrochemistry.68.890
Publication status	Published - 2000 Nov

Keywords

Living Cell
Oxygen Electrode
Scanning Electrochemical Microscopy
Small Volume Analysis

ASJC Scopus subject areas

Electrochemistry

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10.5796/electrochemistry.68.890

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title = "Monitoring Activity of Living Cells Entrapped in a Membrane-Sealed Microcapillary with Scanning Electrochemical Microscopy",

abstract = "The respiration- or photosynthesis-induced change of oxygen concentration was monitored with scanning electrochemical microscopy (SECM). Cell suspension was put into a glass capillary tube and then one edge was sealed with an oxygen permeable membrane. The total volume of the cell suspension in the capillary was as small as 10 μL. The edge of the sealed capillary was imaged with SECM based on oxygen reduction current. The images indicated the respiration activity as low as 2.0×103 HeLa-S3 cells. This method was also applied for imaging the respiration and photosynthesis activities of a single alga (Bryopsis plumosa) protoplast.",

keywords = "Living Cell, Oxygen Electrode, Scanning Electrochemical Microscopy, Small Volume Analysis",

author = "Hitoshi Shiku and Takuo Shiraishi and Hiroaki Ohya and Tomokazu Matsue and Hiroyuki Abe and Hiroyoshi Hoshi and Masato Kobayashi",

year = "2000",

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language = "English",

volume = "68",

pages = "890--892",

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publisher = "Electrochemical Society of Japan",

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T1 - Monitoring Activity of Living Cells Entrapped in a Membrane-Sealed Microcapillary with Scanning Electrochemical Microscopy

AU - Shiku, Hitoshi

AU - Shiraishi, Takuo

AU - Ohya, Hiroaki

AU - Matsue, Tomokazu

AU - Abe, Hiroyuki

AU - Hoshi, Hiroyoshi

AU - Kobayashi, Masato

PY - 2000/11

Y1 - 2000/11

N2 - The respiration- or photosynthesis-induced change of oxygen concentration was monitored with scanning electrochemical microscopy (SECM). Cell suspension was put into a glass capillary tube and then one edge was sealed with an oxygen permeable membrane. The total volume of the cell suspension in the capillary was as small as 10 μL. The edge of the sealed capillary was imaged with SECM based on oxygen reduction current. The images indicated the respiration activity as low as 2.0×103 HeLa-S3 cells. This method was also applied for imaging the respiration and photosynthesis activities of a single alga (Bryopsis plumosa) protoplast.

AB - The respiration- or photosynthesis-induced change of oxygen concentration was monitored with scanning electrochemical microscopy (SECM). Cell suspension was put into a glass capillary tube and then one edge was sealed with an oxygen permeable membrane. The total volume of the cell suspension in the capillary was as small as 10 μL. The edge of the sealed capillary was imaged with SECM based on oxygen reduction current. The images indicated the respiration activity as low as 2.0×103 HeLa-S3 cells. This method was also applied for imaging the respiration and photosynthesis activities of a single alga (Bryopsis plumosa) protoplast.

KW - Living Cell

KW - Oxygen Electrode

KW - Scanning Electrochemical Microscopy

KW - Small Volume Analysis

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SN - 1344-3542

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Monitoring Activity of Living Cells Entrapped in a Membrane-Sealed Microcapillary with Scanning Electrochemical Microscopy

Abstract

Keywords

ASJC Scopus subject areas

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