Monitoring Activity of Living Cells Entrapped in a Membrane-Sealed Microcapillary with Scanning Electrochemical Microscopy

Hitoshi Shiku, Takuo Shiraishi, Hiroaki Ohya, Tomokazu Matsue, Hiroyuki Abe, Hiroyoshi Hoshi, Masato Kobayashi

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

The respiration- or photosynthesis-induced change of oxygen concentration was monitored with scanning electrochemical microscopy (SECM). Cell suspension was put into a glass capillary tube and then one edge was sealed with an oxygen permeable membrane. The total volume of the cell suspension in the capillary was as small as 10 μL. The edge of the sealed capillary was imaged with SECM based on oxygen reduction current. The images indicated the respiration activity as low as 2.0×103 HeLa-S3 cells. This method was also applied for imaging the respiration and photosynthesis activities of a single alga (Bryopsis plumosa) protoplast.

Original languageEnglish
Pages (from-to)890-892
Number of pages3
JournalElectrochemistry
Volume68
Issue number11
DOIs
Publication statusPublished - 2000 Nov

Keywords

  • Living Cell
  • Oxygen Electrode
  • Scanning Electrochemical Microscopy
  • Small Volume Analysis

ASJC Scopus subject areas

  • Electrochemistry

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