Moment selective digital detection of single magnetic beads for multiplexed bioassays

J. Llandro, T. J. Hayward, J. A.C. Bland, D. Morecroft, F. J. Castaño, I. A. Colin, C. A. Ross

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Research into lab-on-a-chip multiplexed bioassays has focused on libraries of biochemical probes, indexed by optically encoded micron-sized labels. However, few current methods have reconciled large multiplexing capability with a rapid detection system amenable to miniaturization. Magnetic identification of labels provides a strong candidate solution to this problem, yet no proposed single-label magnetic detection system can both read and encode magnetic labels. We present a magnetic multiplexed assay in lab-on-a-chip format which identifies target biomolecules from the hybridization results by reading encoded magnetic beads. We show that a microfabricated magnetoresistive ring-shaped sensor can read the magnetic moments of individual commercially available paramagnetic beads using an active digital technique. This work provides proof of principle for a new approach to magnetic labeling of biomolecules for high-throughput bioassays.

Original languageEnglish
Title of host publicationBiomagnetism and Magnetic Biosystems Based on Molecular Recognition Processes
Pages176-185
Number of pages10
DOIs
Publication statusPublished - 2008
Externally publishedYes
EventBiomagnetism and Magnetic Biosystems Based on Molecular Recognition Processes - Sant Feliu de Guixols, Spain
Duration: 2007 Sep 222007 Sep 27

Publication series

NameAIP Conference Proceedings
Volume1025
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Other

OtherBiomagnetism and Magnetic Biosystems Based on Molecular Recognition Processes
CountrySpain
CitySant Feliu de Guixols
Period07/9/2207/9/27

Keywords

  • Bioassay
  • Digital detection
  • Magnetic beads
  • Magnetic labeling
  • Spin-valve ring

ASJC Scopus subject areas

  • Physics and Astronomy(all)

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