TY - JOUR
T1 - Molybdate recovery using immobilized bioengineered Saccharomyces cerevisiae
AU - Stephanie, Audrey
AU - Chien, Mei Fang
AU - Ikeda, Naoya
AU - Inoue, Chihiro
N1 - Funding Information:
This research was supported by the Japan Society for the Promotion of Science (JSPS) KAKENHI Grant Number 18K19042 (Challenging Research (Exploratory)) and 19H02654 (Grant-in-Aid for Scientific Research (B)). This work was partially supported by research grant from the Iwatani Naoji Foundation and Tohoku University Center for Gender Equality Promotion (TUMUG) Support Project (Project to Promote Gender Equality and Female Researchers). The authors gratefully thank Dr. Masaoki Uno for his assistance in ICP-MS measurement during kinetic analysis.
PY - 2020/12
Y1 - 2020/12
N2 - Molybdenum, a precious metal with important roles in industries, is projected to experience future shortage. Despite this, there is still no practical recycling process of this metal and around 25 thousand tons of molybdenum are discharged into wastewater annually. Biorecovery is a promising approach to resolve the above problem because of its high selectivity, high sensitivity, low running cost and low environmental burden. A bacterial molybdenum binding protein ModE has been constructed into yeast cells and named as ScBp5. In this study, the potential of genetic engineered yeast for recovering molybdenum from wastewater was further investigated. In order to stabilize the expression of ModE, strain ScBp5 was further modified by replacing promoter upstream of modE to generate ScBp6. The improvement of molybdenum adsorption efficiency by ScBp6 was confirmed especially in low concentration. The molybdenum isotherms parameters of ScBp6 was analyzed. To enable the usage as biorecovery agent in industrial settings, ScBp6 cells were immobilized using calcium alginate matrix, and the optimum immobilization conditions for ScBp6 were determined as 2% matrix density, 4 h of immobilization time, and 10 mg/ml cell density. The investigation of molybdenum adsorption kinetics by immobilized ScBp6 cells showed this adsorption was an efficient chemisorption. Overall, this research demonstrate the efficiency of immobilized yeast cell in molybdenum recovery.
AB - Molybdenum, a precious metal with important roles in industries, is projected to experience future shortage. Despite this, there is still no practical recycling process of this metal and around 25 thousand tons of molybdenum are discharged into wastewater annually. Biorecovery is a promising approach to resolve the above problem because of its high selectivity, high sensitivity, low running cost and low environmental burden. A bacterial molybdenum binding protein ModE has been constructed into yeast cells and named as ScBp5. In this study, the potential of genetic engineered yeast for recovering molybdenum from wastewater was further investigated. In order to stabilize the expression of ModE, strain ScBp5 was further modified by replacing promoter upstream of modE to generate ScBp6. The improvement of molybdenum adsorption efficiency by ScBp6 was confirmed especially in low concentration. The molybdenum isotherms parameters of ScBp6 was analyzed. To enable the usage as biorecovery agent in industrial settings, ScBp6 cells were immobilized using calcium alginate matrix, and the optimum immobilization conditions for ScBp6 were determined as 2% matrix density, 4 h of immobilization time, and 10 mg/ml cell density. The investigation of molybdenum adsorption kinetics by immobilized ScBp6 cells showed this adsorption was an efficient chemisorption. Overall, this research demonstrate the efficiency of immobilized yeast cell in molybdenum recovery.
KW - Cell-surface display biorecovery
KW - Immobilization
KW - Molybdate
KW - Rare metal
KW - Saccharomyces cerevisiae
UR - http://www.scopus.com/inward/record.url?scp=85092246131&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85092246131&partnerID=8YFLogxK
U2 - 10.1016/j.hydromet.2020.105491
DO - 10.1016/j.hydromet.2020.105491
M3 - Article
AN - SCOPUS:85092246131
VL - 198
JO - Hydrometallurgy
JF - Hydrometallurgy
SN - 0304-386X
M1 - 105491
ER -