Molecular viewing of actin polymerizing actions and beyond: Combination analysis of single-molecule speckle microscopy with modeling, FRAP and s-FDAP (sequential fluorescence decay after photoactivation)

Naoki Watanabe, Sawako Yamashiro, Dimitrios Vavylonis, Tai Kiuchi

    Research output: Contribution to journalReview articlepeer-review

    5 Citations (Scopus)

    Abstract

    Live-cell single-molecule imaging is a powerful tool to elucidate the in vivo biochemistry of cytoskeletal proteins. However, it is often somewhat difficult to interpret how a bulk population of the observed molecule might behave as a whole. We review our recent studies in which the combination of image analysis with modeling and bulk kinetics measurements such as FRAP (fluorescence recovery after photobleaching) clarified basic problems in the regulation of actin remodeling pathways. Development, Growth & Differentiation

    Original languageEnglish
    Pages (from-to)508-514
    Number of pages7
    JournalDevelopment Growth and Differentiation
    Volume55
    Issue number4
    DOIs
    Publication statusPublished - 2013 May

    Keywords

    • Actin turnover
    • Dual-color FRAP
    • Formin homology proteins
    • S-FDAP
    • Single-molecule speckle microscopy

    ASJC Scopus subject areas

    • Developmental Biology
    • Cell Biology

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