Molecular systematics of Volvocales (Chlorophyceae, Chlorophyta) based on exhaustive 18S rRNA phylogenetic analyses

Takashi Nakada, Kazuharu Misawa, Hisayoshi Nozaki

Research output: Contribution to journalArticle

111 Citations (Scopus)

Abstract

The taxonomy of Volvocales (Chlorophyceae, Chlorophyta) was traditionally based solely on morphological characteristics. However, because recent molecular phylogeny largely contradicts the traditional subordinal and familial classifications, no classification system has yet been established that describes the subdivision of Volvocales in a manner consistent with the phylogenetic relationships. Towards development of a natural classification system at and above the generic level, identification and sorting of hundreds of sequences based on subjective phylogenetic definitions is a significant step. We constructed an 18S rRNA gene phylogeny based on 449 volvocalean sequences collected using exhaustive BLAST searches of the GenBank database. Many chimeric sequences, which can cause fallacious phylogenetic trees, were detected and excluded during data collection. The results revealed 21 strongly supported primary clades within phylogenetically redefined Volvocales. Phylogenetic classification following PhyloCode was proposed based on the presented 18S rRNA gene phylogeny along with the results of previous combined 18S and 26S rRNA and chloroplast multigene analyses.

Original languageEnglish
Pages (from-to)281-291
Number of pages11
JournalMolecular Phylogenetics and Evolution
Volume48
Issue number1
DOIs
Publication statusPublished - 2008 Jul

Keywords

  • 18S rRNA
  • BLAST
  • Chimeric sequence
  • Chlorophyceae
  • PhyloCode
  • Systematics
  • Volvocales

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics

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