Depletion of the stratospheric ozone layer leads to an increase in ambient UV loads, which are expected to raise skin cancer incidences. Tumor development in the skin could be a multistep process in which various genetic alterations, such as point mutations and deletions, occur successively. Here, we demonstrate that UVB irradiation efficiently induces deletions in the epidermis using a novel transgenic mouse, gpt delta. In this mouse model, deletions in λ DNA integrated in the chromosome are preferentially selected as Spi- (sensitive to P2 interference) phages, which can then be subjected to molecular analysis. The mice were exposed to UVB at single doses of 0.3, 0.5, 1.0, 1.5, and 2.0 kJ/m2. After 4 weeks, λ phage was rescued from the genomic DNA of the epidermis by in vitro packaging reactions. The mutant frequencies of Spi- with large deletions in the epidermis increased >15-fold at a UVB dose of 0.5 kJ/m2 over the control. Molecular sizes of most of the large deletions were >1000 bp. More than one-half of the large deletions occurred between short direct-repeat sequences from 1 to 6 bp, and the remainder had flush ends. In the unirradiated mouse, almost all of the Spi- mutants were 1-bp frameshifts in runs of identical bases. These results suggest that UVB irradiation induces deletions in the murine epidermis, and most of the deletions are generated through end-joining of double strand breaks in DNA.
|Number of pages||6|
|Publication status||Published - 2001 May 15|
ASJC Scopus subject areas
- Cancer Research