TY - JOUR
T1 - Molecular integration on phospholipid polymer-coated magnetic beads for gene expression analysis in cells
AU - Ishihara, Kazuhiko
AU - Fukuda, Yoshito
AU - Konno, Tomohiro
AU - Inoue, Yuuki
N1 - Funding Information:
This research was supported by a Grant-in-Aid for Scientific Research on Innovative Areas “Nanomedicine Molecular Science” (No. 2306 ), Ministry of Education, Culture, Sports, Science, and Technology of Japan.
Publisher Copyright:
© 2017 Elsevier B.V.
PY - 2017/10
Y1 - 2017/10
N2 - Magnetic polymer beads, composed of a polystyrene core and hydrophilic poly(2-methacryloyloxyethyl phosphorylcholine (MPC)) graft layer, containing magnetic nanoparticles were prepared for analyzing genes in the cells. The initiator group for atom transfer radical polymerization (ATRP) was provided on the surface of the beads and polymerization of MPC created the poly(MPC) graft layer. The terminal bromine group of poly(MPC) chains converted to a reactive group. Streptavidin was immobilized using the terminal reactive group in the poly(MPC) chains for capturing the biotinated DNA primer of the polymerase chain reaction (PCR). After selective binding of three kinds of messenger RNA from the cell lysate, PCR was carried out to increase complementary DNA. The polymer beads were stable even under the PCR thermal cycling conditions, and dispersed again easily. The magnetic polymer beads can be candidate solid support for PCR of cell lysates.
AB - Magnetic polymer beads, composed of a polystyrene core and hydrophilic poly(2-methacryloyloxyethyl phosphorylcholine (MPC)) graft layer, containing magnetic nanoparticles were prepared for analyzing genes in the cells. The initiator group for atom transfer radical polymerization (ATRP) was provided on the surface of the beads and polymerization of MPC created the poly(MPC) graft layer. The terminal bromine group of poly(MPC) chains converted to a reactive group. Streptavidin was immobilized using the terminal reactive group in the poly(MPC) chains for capturing the biotinated DNA primer of the polymerase chain reaction (PCR). After selective binding of three kinds of messenger RNA from the cell lysate, PCR was carried out to increase complementary DNA. The polymer beads were stable even under the PCR thermal cycling conditions, and dispersed again easily. The magnetic polymer beads can be candidate solid support for PCR of cell lysates.
KW - Bioaffinity
KW - Dispersibility
KW - Magnetic beads
KW - Phospholipid polymer
KW - Thermal stability
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U2 - 10.1016/j.reactfunctpolym.2017.08.011
DO - 10.1016/j.reactfunctpolym.2017.08.011
M3 - Article
AN - SCOPUS:85028919288
SN - 1381-5148
VL - 119
SP - 125
EP - 133
JO - Reactive and Functional Polymers
JF - Reactive and Functional Polymers
ER -